Yang Chung-Yao, Chang Ting-Chang, Lee Yi-Tzu, Shih Ting-Ying, Li Chang-Wei, Cheng Chao-Min
Hygeia Touch Inc., Taipei, Taiwan.
Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Linkou branch, Taoyuan, Taiwan.
J Med Virol. 2025 Feb;97(2):e70190. doi: 10.1002/jmv.70190.
This study examined the relationship between the vaginal microbiome, HPV infection, and cervical intraepithelial neoplasia (CIN) in 173 women. Subjects were grouped by HPV status and cervical lesion severity, ranging from HPV-negative to CIN Grade 2 or higher. Using VALENCIA classification, the study identified different community state types (CSTs) of vaginal microbiota, with CST IV subtypes (Staphylococcus dominated) showing high diversity and increased pathogenic bacteria. Results showed a higher microbial diversity in women with CIN compared to HPV-negative women, with specific microbial profiles like Enterococcus faecalis and Staphylococcus aureus correlating with HPV infection severity. LEfSe and STAMP analyses revealed that particular bacterial species, such as Fannyhessea vaginae and Sneathia vaginalis, could serve as biomarkers for HPV and CIN status. Furthermore, Streptococcus and Staphylococcus were associated with HPV genotypes α7 and α9, respectively. These findings highlight how the composition of vaginal microbiota may influence HPV persistence and CIN progression, emphasizing the need for further research into microbial dysbiosis as a potential target for cervical cancer prevention and management.
本研究调查了173名女性的阴道微生物群、人乳头瘤病毒（HPV）感染与宫颈上皮内瘤变（CIN）之间的关系。研究对象按HPV状态和宫颈病变严重程度分组，范围从HPV阴性到CIN 2级或更高。该研究使用巴伦西亚分类法，确定了阴道微生物群的不同群落状态类型（CSTs），其中CST IV亚型（以葡萄球菌为主）显示出高多样性和病原菌增加。结果显示，与HPV阴性女性相比，CIN女性的微生物多样性更高，粪肠球菌和金黄色葡萄球菌等特定微生物谱与HPV感染严重程度相关。线性判别分析效应大小（LEfSe）和统计分析微生物谱（STAMP）分析表明，特定细菌物种，如阴道芬尼酵母和阴道斯奈氏菌，可作为HPV和CIN状态的生物标志物。此外，链球菌和葡萄球菌分别与HPV基因型α7和α9相关。这些发现凸显了阴道微生物群的组成可能如何影响HPV持续感染和CIN进展，强调了有必要进一步研究微生物失调作为宫颈癌预防和管理潜在靶点的可能性。
