Department of Obstetrics & Gynecology, Zhangpu Hospital, Zhangzhou, China.
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Front Cell Infect Microbiol. 2022 Feb 14;12:767693. doi: 10.3389/fcimb.2022.767693. eCollection 2022.
Cervical intraepithelial neoplasia (CIN) is a precancerous condition inducing local lesions on the surface of the squamocolumnar junction of the cervix. Despite the role of vaginal microbiota having been under-discussed, the role of the cervical microbiome and the microbial migration across the reproductive tract involved in CIN was limitedly studied. We aimed to synchronously characterize the dysbiosis associated with CIN in both the cervix and vagina in a Chinese population. Profiling of cervical and vaginal microbiota from 60 CIN women and 60 healthy women was conducted. 16S rRNA sequencing was adopted. By comparing the microbial profiles between different parts of the reproductive tract, our results demonstrated an increased shift of microbial diversity in the cervix compared with that in the vagina for the CIN patients, specifically in CIN 1. Less dysbiosis was found between the CIN patients and controls, in either the vagina or cervix. The microbial community may be modulated by the onset of sexual activity, a known clinical risk factor for cervical neoplasia. Distinct patterns of perturbated bacteria were found in the vaginal and cervical microbiota, in which reduced Actinobacteria-related operational taxonomic units (OTUs) and increased Proteobacteria-related OTUs were found in the vagina and cervix, respectively. A good agreement between the direction of the top-significant perturbated OTUs was observed between the vaginal and cervical microbiome, suggesting a potential microbial migration in the reproductive tract. Enriched genera such as and were found in cervical microbiota-associated CIN. Multivariate analysis revealed , , and as independent genera contributing to CIN in the cervix. In summary, this study revealed the perturbation of microbiota in the presence of CIN and demonstrated a distinct pattern of characteristic bacteria community between the vagina and cervix involved in the development of CIN.
宫颈上皮内瘤变（CIN）是一种癌前病变，会在宫颈鳞柱状交界表面引起局部损伤。尽管阴道微生物群的作用尚未得到充分讨论，但宫颈微生物组以及微生物在生殖道中的迁移在 CIN 中的作用研究有限。我们旨在同步描述中国人群中 CIN 相关的宫颈和阴道菌群失调。对 60 名 CIN 患者和 60 名健康女性的宫颈和阴道微生物组进行了分析。采用 16S rRNA 测序。通过比较生殖道不同部位的微生物谱，我们的研究结果表明，与健康对照组相比，CIN 患者的宫颈微生物多样性增加，尤其是在 CIN1 中。在阴道或宫颈中，CIN 患者和对照组之间的失调程度较小。微生物群可能受到性活动的影响，性活动是宫颈癌的一个已知临床危险因素。在阴道和宫颈微生物群中发现了不同的细菌失调模式，其中阴道和宫颈中的放线菌相关操作分类单元（OTU）减少，而变形菌相关 OTU 增加。阴道和宫颈微生物群中发现了显著失调的 OTU 方向之间存在良好的一致性，表明生殖道中可能存在微生物迁移。在与 CIN 相关的宫颈微生物群中发现了丰富的属，如 和 。多变量分析显示 、 和 是导致宫颈 CIN 的独立菌属。总之，本研究揭示了 CIN 存在时微生物群的失调，并显示了阴道和宫颈之间涉及 CIN 发展的特征性细菌群落的独特模式。
