Goodbourn S, Zinn K, Maniatis T
Cell. 1985 Jun;41(2):509-20. doi: 10.1016/s0092-8674(85)80024-6.
We have localized the regulatory sequence required for viral or poly(I)-poly(C) activation of human beta-interferon gene expression to a region located between -37 and -77 from the mRNA cap site. This sequence has the characteristics of an inducible enhancer element: it can act upstream or downstream of the beta-interferon gene regardless of its orientation, and at distances up to approximately 1 kilobase from its normal location. Moreover, this element can confer inducibility on a heterologous promoter. Further analysis has identified a minimal regulatory element of 14 base pairs within this enhancer. Sequences closely related to this element are present five times within the 5'-flanking regions of both the alpha- and beta-interferon genes. The number of these minimal regulatory elements required for maximal beta-interferon gene expression appears to differ in different cell lines.
我们已将病毒或聚肌苷酸-聚胞苷酸（poly(I)-poly(C)）激活人β-干扰素基因表达所需的调控序列定位到距mRNA帽位点-37至-77之间的区域。该序列具有可诱导增强子元件的特征：无论其方向如何，它都可以在β-干扰素基因的上游或下游起作用，并且距离其正常位置可达约1千碱基。此外，该元件可赋予异源启动子可诱导性。进一步的分析已在该增强子内鉴定出一个14个碱基对的最小调控元件。与该元件密切相关的序列在α-和β-干扰素基因的5'侧翼区域内均出现了五次。在不同细胞系中，实现最大β-干扰素基因表达所需的这些最小调控元件的数量似乎有所不同。
