Fujita T, Ohno S, Yasumitsu H, Taniguchi T
Cell. 1985 Jun;41(2):489-96. doi: 10.1016/s0092-8674(85)80022-2.
The human interferon-beta (IFN-beta) gene contains sequences within its 5'-flanking region that mediate the virus-induced activation of mRNA transcription. A series of mutant IFN-beta genes, including the 5' deletion mutants, was constructed and introduced into mouse L929 cells. Expression study of those mutant genes demonstrates that: sequences upstream, but not downstream, of -40 from the cap site are responsible for the viral induction of the gene; that the upstream boundary of the DNA sequences required to support the maximum level of induction lies between -117 and -105 from the cap site; and that this upstream sequence shows a property similar to enhancer elements as it functions in either orientation with a latitude in distance from the cap site. Within this sequence, we note the presence of repetitious hexanucleotides (consensus: A-A-AG-TG-G-A), each of which may play a role in the maximum induction of the IFN-beta gene.
人类β干扰素（IFN-β）基因在其5'侧翼区域包含介导病毒诱导mRNA转录激活的序列。构建了一系列突变的IFN-β基因，包括5'缺失突变体，并将其导入小鼠L929细胞。对这些突变基因的表达研究表明：帽位点-40上游而非下游的序列负责该基因的病毒诱导；支持最大诱导水平所需的DNA序列的上游边界位于帽位点-117至-105之间；并且该上游序列显示出与增强子元件类似的特性，因为它在与帽位点的距离上具有一定灵活性的任何方向上都能发挥作用。在该序列中，我们注意到存在重复的六核苷酸（共有序列：A-A-AG-TG-G-A），其中每一个可能在IFN-β基因的最大诱导中发挥作用。
