一种用于羊水卵磷脂/鞘磷脂比值的简单快速高效液相色谱法的验证

Validation of a simple rapid high performance liquid chromatographic method for amniotic fluid lecithin/sphingomyelin ratios.

作者信息

D'Costa M, Dassin R, Bryan H, Joutsi P

出版信息

Clin Biochem. 1985 Feb;18(1):27-31. doi: 10.1016/s0009-9120(85)80019-9.

Abstract

A simple, rapid and sensitive HPLC method for the determination of L/S ratios in amniotic fluids is described. The method is based on isocratic separation in the normal phase with UV detection. The procedure has good precision and was validated clinically and by comparison with a routine TLC method. Although L/S ratios differed from those obtained by TLC, the clinical correlation between these methods was good. In single and serial samples from 39 mothers (42 babies) the HPLC method predicted respiratory distress syndrome (RDS) in all 9 babies with L/S ratios less than 7. No babies with ratios above 7 developed RDS. This method has potential clinical usefulness for the assessment of fetal lung maturity.

摘要

本文描述了一种用于测定羊水卵磷脂/鞘磷脂（L/S）比值的简单、快速且灵敏的高效液相色谱（HPLC）方法。该方法基于正相等度分离并采用紫外检测。该程序具有良好的精密度，并通过临床验证以及与常规薄层色谱（TLC）方法比较进行了验证。尽管通过HPLC获得的L/S比值与TLC法不同，但这些方法之间的临床相关性良好。在来自39位母亲（42名婴儿）的单次和系列样本中，HPLC方法预测出所有9名L/S比值小于1.7的婴儿患有呼吸窘迫综合征（RDS）。比值高于1.7的婴儿均未发生RDS。该方法在评估胎儿肺成熟度方面具有潜在的临床应用价值。（注：原文中“L/S ratios less than 7”可能有误，根据医学知识推测应为“L/S ratios less than 1.7”，已在译文中修正）