Ng H T, Hsu L M, Leung W Y, Chen C Y
Proc Natl Sci Counc Repub China B. 1987 Apr;11(2):91-6.
One hundred amniotic fluid (AF) specimens of women at 28 to 37 weeks gestation were obtained during labor for lecithin/sphingomyelin ratio (L/S ratio) study by using high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). The results are divided into two groups: one with L/S ratio at or more than 2, and the other with L/S ratio of less than 2. Of the 80 measured by HPLC with the ratio of above 2 or 1.3%, one suffered respiratory distress syndrome (RDS); of the 65 measured by TLC also with the ratio of above 2 or 3.1%, two developed RDS. The result bears no great significance here. However, in the groups with L/S ratio of less than two, the results were very significant: 17 of 20 estimated by HPLC happened to have RDS for an incidence of false positive rate of 15% compared with a 54.3% in 16 of the 35 women evaluated by TLC.
在分娩期间，收集了100例妊娠28至37周女性的羊水样本，通过高效液相色谱法（HPLC）和薄层色谱法（TLC）进行卵磷脂/鞘磷脂比值（L/S比值）研究。结果分为两组：一组L/S比值等于或大于2，另一组L/S比值小于2。在通过HPLC测量的80例L/S比值高于2的样本中，有1例（占1.3%）发生呼吸窘迫综合征（RDS）；在通过TLC测量的65例L/S比值高于2的样本中，有2例（占3.1%）发生RDS。在此处该结果无重大意义。然而，在L/S比值小于2的组中，结果非常显著：通过HPLC评估的20例中有17例发生RDS，假阳性率为15%，而通过TLC评估的35例中的16例发生率为54.3%。
