Zhang Zaimei, Li Hui, Han Han, Qin Lijian, Lu Wei, Yue Lin, Guo Zongzhen, Gao Shengsong, Chen Shuang, Liu Huie, Wang Dong, Wang Jiqian
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.
State Key Laboratory of Heavy Oil Processing and Centre for Bioengineering and Biotechnology, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.
J Environ Manage. 2025 Feb;375:124298. doi: 10.1016/j.jenvman.2025.124298. Epub 2025 Jan 26.
Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants with mutagenicity, carcinogenicity and teratogenicity, widely distributed in the environment. Effective biodegradation of PAHs is highly required, especially in wastewater. An efficient PAHs degrading strain Streptomyces sp. M-1 was isolated from polluted kerosene. The degradation capacity of anthracene and phenanthrene was evaluated under various PAHs concentrations, pH, and temperatures by M-1. To find the degradation pathways, the key intermediates were detected by mass spectrometry and the enzyme-encoding genes were analyzed by many bioinformatics tools. Furthermore, the potential of the strain for bioremediation in PAH-contaminated water was evaluated. The results showed that the maximal degradation rate of anthracene and phenanthrene reached 93.14% (100 mg L, 7 days) and 49.25% (50 mg L, 7 days), respectively. Their average degradation rate increased within the concentration of 50-800 mg L and reached 2.72 mg d for anthracene and 1.28 mg d for phenanthrene at 800 mg L. M-1 exhibited high and stable anthracene degradation rate under tested pH and temperatures, and high phenanthrene degradation under tested pH and higher temperatures. Based on the analysis of both intermediates and enzyme-encoding genes, it is proposed that anthracene undergoes degradation via the phthalic acid pathway, while phenanthrene follows the salicylic acid pathway. Finally, 98.98% degradation of anthracene and 72.77% degradation of phenanthrene in water was realized over 14 days. We thus propose that Streptomyces sp. M-1 is an effective degrader for bioremediation of PAHs pollution.
多环芳烃（PAHs）是具有致突变性、致癌性和致畸性的持久性有机污染物，广泛分布于环境中。迫切需要对PAHs进行有效的生物降解，尤其是在废水中。从受污染的煤油中分离出了一株高效的PAHs降解菌株链霉菌属M-1。通过M-1在不同的PAHs浓度、pH值和温度条件下评估了其对蒽和菲的降解能力。为了找到降解途径，通过质谱检测关键中间体，并使用多种生物信息学工具分析酶编码基因。此外，还评估了该菌株在PAH污染水体中的生物修复潜力。结果表明，蒽和菲的最大降解率分别达到93.14%（100 mg/L，7天）和49.25%（50 mg/L，7天）。在50-800 mg/L的浓度范围内，它们的平均降解率有所增加，在800 mg/L时，蒽的平均降解率达到2.72 mg/d，菲的平均降解率达到1.28 mg/d。M-1在测试的pH值和温度条件下对蒽表现出高且稳定的降解率，在测试的pH值和较高温度下对菲表现出较高的降解率。基于对中间体和酶编码基因的分析，提出蒽通过邻苯二甲酸途径进行降解，而菲则遵循水杨酸途径。最终，在14天内实现了水中98.98%的蒽降解和72.77%的菲降解。因此，我们认为链霉菌属M-1是PAHs污染生物修复的有效降解菌。
