Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen, China.
Xiamen Key Laboratory of Marine Genetic Resources, State Key Laboratory Breeding Base, Xiamen, China.
Appl Environ Microbiol. 2018 Oct 17;84(21). doi: 10.1128/AEM.01261-18. Print 2018 Nov 1.
Bacteria play an important role in the removal of polycyclic aromatic hydrocarbons (PAHs) from polluted environments. In marine environments, is one of the most prevalent PAH-degrading bacterial genera. However, little is known regarding the degradation mechanisms for multiple PAHs by sp. strain P1 was isolated from deep-sea sediments and is known to degrade naphthalene, phenanthrene, pyrene, and other aromatic hydrocarbons. Here, six ring-hydroxylating dioxygenases (RHDs) were identified in the complete genome of sp. P1 and were confirmed to be involved in PAH degradation by enzymatic assays. Further, five gene clusters in its genome were identified to be responsible for PAH degradation. Degradation pathways for naphthalene, phenanthrene, and pyrene were elucidated in sp. P1 based on genomic and transcriptomic analysis and characterization of an interconnected metabolic network. The metabolic pathway overlaps in many steps in the degradation of pyrene, phenanthrene, and naphthalene, which were validated by the detection of metabolic intermediates in cultures. This study describes a pyrene degradation pathway for Moreover, the study represents the integration of a PAH metabolic network that comprises pyrene, phenanthrene, and naphthalene degradation pathways. Taken together, these results provide a comprehensive investigation of PAH metabolism in PAHs are ubiquitous in the environment and are carcinogenic compounds and tend to accumulate in food chains due to their low bioavailability and poor biodegradability. is an obligate marine PAH degrader and is widespread in marine environments, while the PAH degradation pathways remain unclear. In this report, the degradation pathways for naphthalene, phenanthrene, and pyrene were revealed, and an integrated PAH metabolic network covering pyrene, phenanthrene, and naphthalene was constructed in This overlapping network provides streamlined processing of PAHs to intermediates and ultimately to complete mineralization. Furthermore, these results provide an additional context for the prevalence of in oil-polluted marine environments and pelagic settings. In conclusion, these analyses provide a useful framework for understanding the cellular processes involved in PAH metabolism in an ecologically important marine bacterium.
细菌在去除受污染环境中的多环芳烃（PAHs）方面发挥着重要作用。在海洋环境中，假单胞菌属是最常见的 PAH 降解细菌属之一。然而，关于 sp. 菌株 P1 从深海沉积物中分离出来，已知可降解萘、菲、芘和其他芳烃。在这里，在 sp. P1 的完整基因组中鉴定出了 6 种环羟基化双加氧酶（RHD），并通过酶促测定证实它们参与了 PAH 的降解。此外，在其基因组中鉴定出 5 个基因簇负责 PAH 的降解。基于基因组和转录组分析以及相互关联的代谢网络的表征，阐明了 sp. P1 中萘、菲和芘的降解途径。在培养物中检测到代谢中间产物，验证了多环芳烃降解途径在 sp. P1 中重叠。这项研究描述了 sp. P1 中芘的降解途径。此外，该研究代表了包括芘、菲和萘降解途径在内的 PAH 代谢网络的整合。总之，这些结果提供了对 sp. P1 中 PAH 代谢的全面研究。PAHs 在环境中普遍存在，是致癌化合物，由于其低生物利用度和较差的生物降解性，往往会在食物链中积累。sp. P1 是一种专性海洋 PAH 降解菌，广泛存在于海洋环境中，而其 PAH 降解途径尚不清楚。在本报告中，揭示了萘、菲和芘的降解途径，并在 sp. P1 中构建了一个涵盖芘、菲和萘的综合 PAH 代谢网络。这个重叠的网络为 PAHs 到中间体再到完全矿化的处理提供了流线型的处理。此外，这些结果为 sp. P1 在受石油污染的海洋环境和远洋环境中的流行提供了额外的背景。总之，这些分析为理解海洋中一种重要的细菌中 PAH 代谢所涉及的细胞过程提供了一个有用的框架。
