Ma Yanwei, Zuohereguli Kuerban, Zhang Lisheng, Kang Yalong, Shi Liwen, Xu Hao, Ruan Yang, Wen Tao, Mei Xinlan, Dong Caixia, Xu Yangchun, Shen Qirong
Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Key Lab of Organic-Based Fertilizers of China, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Nanjing Agricultural University, Nanjing, China.
School of Ecology and Environmental Sciences, Yunnan University, Kunming, China.
Plant Cell Environ. 2025 Jun;48(6):3968-3980. doi: 10.1111/pce.15395. Epub 2025 Jan 27.
Bacillus velezensis SQR9 or Trichoderma harzianum NJAU4742-amended bioorganic fertilizers might significantly improve the soil microbial community and crop yields. However, the mechanisms these microorganisms act are far away from distinctness. We combined amplicon sequencing with culturable approaches to investigate the effects of these microorganisms on pear tree growth, rhizosphere nutrients and microbial mechanisms. The SQR9 and T4742 treatments increased the total biomass of pear trees by 68% and 84%, respectively, compared to the conventional organic fertilizer treatment (CK). SQR9 tends to increase soil organic matter and available phosphorus, while T4742 more effectively enhances nitrogen, potassium, iron and zinc levels. These effects were primarily linked to changes in the microbial community. T4742 treatment enriched twice as many differential microbes as SQR9. SQR9 significantly enriched Urebacillus, Streptomyces and Mycobacterium, while T4742 increased the abundance of Pseudomonas, Aspergillus and Penicillium. In vitro experiments revealed that secondary metabolites secreted by B. velezensis SQR9 and T. harzianum NJAU4742 stimulate the growth of key probiotics associated with their respective treatments, enhancing soil fertility and plant biomass. The study revealed the specific roles of these bioorganic fertilizers in agricultural applications, providing new insights for developing effective and targeted bioorganic fertilizer products and promoting sustainable agriculture.
解淀粉芽孢杆菌SQR9或哈茨木霉NJAU4742改良的生物有机肥可能会显著改善土壤微生物群落和作物产量。然而，这些微生物发挥作用的机制远未明晰。我们将扩增子测序与可培养方法相结合，以研究这些微生物对梨树生长、根际养分和微生物机制的影响。与传统有机肥处理（CK）相比，SQR9和T4742处理分别使梨树总生物量增加了68%和84%。SQR9倾向于增加土壤有机质和有效磷，而T4742更有效地提高氮、钾、铁和锌的含量。这些影响主要与微生物群落的变化有关。T4742处理富集的差异微生物数量是SQR9的两倍。SQR9显著富集了脲芽孢杆菌、链霉菌和分枝杆菌，而T4742增加了假单胞菌、曲霉和青霉的丰度。体外实验表明，解淀粉芽孢杆菌SQR9和哈茨木霉NJAU4742分泌的次生代谢产物刺激了与其各自处理相关的关键益生菌的生长，提高了土壤肥力和植物生物量。该研究揭示了这些生物有机肥在农业应用中的具体作用，为开发有效且有针对性的生物有机肥产品和促进可持续农业提供了新的见解。
