Aung Lwin Moe, Renn Ting-Yi, Chin-Yi Lin Jerry, Salamanca Eisner, Wu Yi-Fan, Pan Yu-Hwa, Teng Nai-Chia, Huang Haw-Ming, Sun Ying-Sui, Chang Wei-Jen
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, United States.
J Dent Sci. 2025 Jan;20(1):178-188. doi: 10.1016/j.jds.2024.08.011. Epub 2024 Aug 29.
BACKGROUND/PURPOSE: Osseointegration potential is greatly depended on the interaction between bone cells and dental implant surface. Since zirconia ceramic has a bioinert surface, functionalization of the surface with an organic compound allylamine was conducted to overcome its drawback of minimal interaction with the surrounding bone.
The zirconia surface was initially treated with argon glow discharge plasma (GDP), then combined with amine plasma at three different conditions of 50-W, 75-W and 85-W, to prepare the final samples. The surface characteristics and cell biocompatibility were then evaluated.
Surface morphology analysis revealed a bulbous pattern on allylamine-treated sample groups. The aromatic C-H, C-O, N-H, C ˆ C, and C-H stretching and functional groups have been identified. Surface roughness increased, and hydrophilicity improved after surface modification. Cell viability analysis showed the highest result for the allylamine 50-W (A50) group. Alkaline phosphatase (ALP) assay indicated the A50 group had the highest activity, subsequently promoting late-stage mineralization at day 21. The reverse transcription-quantitative polymerase chain reaction (RT-qPCR) data demonstrated a significant upregulation of osteogenic gene expressions from day 1 to day 21.
The allylamine-treated surface demonstrates immense enhancement in the surface hydrophilicity as well as in the viability, differentiation, and osteogenic properties of osteoblast-like cells. This makes it a promising candidate for future dental implant applications.
背景/目的：骨结合潜力在很大程度上取决于骨细胞与牙种植体表面之间的相互作用。由于氧化锆陶瓷表面具有生物惰性，因此用有机化合物烯丙胺对其表面进行功能化处理，以克服其与周围骨组织相互作用极小的缺点。
氧化锆表面先用氩辉光放电等离子体（GDP）处理，然后在50W、75W和85W三种不同条件下与胺等离子体结合，制备最终样品。随后对表面特性和细胞生物相容性进行评估。
表面形态分析显示烯丙胺处理的样品组呈现球状图案。已鉴定出芳香族C-H、C-O、N-H、C═C和C-H伸缩振动以及官能团。表面改性后表面粗糙度增加，亲水性提高。细胞活力分析显示烯丙胺50W（A50）组的结果最高。碱性磷酸酶（ALP）检测表明A50组活性最高，随后在第21天促进晚期矿化。逆转录定量聚合酶链反应（RT-qPCR）数据表明，从第1天到第21天，成骨基因表达显著上调。
烯丙胺处理的表面在表面亲水性以及成骨样细胞的活力、分化和成骨特性方面显示出极大的增强。这使其成为未来牙种植体应用的有希望的候选材料。
