Yu Wanqi, Wang Xiaona, Guo Yu, Yang Shihui, Zhou Zhe, Sun Xiaolong, Zhang Ruwen, Guo Tianqi, Zhou Yanmin, Zhao Jinghui
Department of Dental Implantology, Hospital of Stomatology, Jilin University, Changchun 130021, People's Republic of China. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun 130021, People's Republic of China.
Biomed Mater. 2020 Aug 28;15(5):055012. doi: 10.1088/1748-605X/ab9096.
Titanium (Ti) and its alloys are widely used in dental implants due to their favorable mechanical properties and biocompatibility. Surface characteristics, including physical and chemical properties, are crucial factors to enhance the osteogenesis performance of Ti. The aim of this study is to evaluate amino group surface modification to facilitate the osteogenic potential and bone repair of dental implants both in vitro and in vivo. Herein, amino group-modified Ti surfaces were constructed via the plasma-enhanced chemical vapor deposition (PECVD) technique with an allylamine monomer. The adhesion, proliferation, alkaline phosphate activity and osteogenesis-related genetic expression of MG-63 cells on the surfaces were performed in vitro and presented a significant increase in amino group-modified Ti compared with that in Ti. The in vivo study in miniature pigs was evaluated through micro-computed tomography analysis and histological evaluation, which exhibited enhanced new bone formation in amino group-modified Ti compared with that in Ti after implantation for 4, 8 and 12 weeks. Consequently, amino group surface modification with the PECVD technique may provide a promising modification method to enhance the osteogenesis performance of Ti implants.
钛（Ti）及其合金因其良好的机械性能和生物相容性而被广泛应用于牙科植入物。包括物理和化学性质在内的表面特性是增强钛骨生成性能的关键因素。本研究的目的是评估氨基表面改性，以促进牙科植入物在体外和体内的成骨潜力及骨修复。在此，通过等离子体增强化学气相沉积（PECVD）技术，以烯丙胺单体构建了氨基改性的钛表面。体外研究了MG-63细胞在这些表面上的粘附、增殖、碱性磷酸酶活性和成骨相关基因表达，结果表明与钛表面相比，氨基改性钛表面上的这些指标均显著增加。通过微型计算机断层扫描分析和组织学评估对小型猪进行了体内研究，结果显示植入4、8和12周后，与钛相比，氨基改性钛表面的新骨形成增强。因此，采用PECVD技术进行氨基表面改性可能为增强钛植入物的成骨性能提供一种有前景的改性方法。
