Rohr Nadja, Fricke Katja, Bergemann Claudia, Nebe J Barbara, Fischer Jens
Biomaterials and Technology, Department of Reconstructive Dentistry, University Center for Dental Medicine, University of Basel, 4058 Basel, Switzerland.
Department of Cell Biology, Rostock University Medical Center, 18057 Rostock, Germany.
J Clin Med. 2020 Aug 27;9(9):2776. doi: 10.3390/jcm9092776.
Plasma-polymerized allylamine (PPAAm) coatings of titanium enhance the cell behavior of osteoblasts. The purpose of the present study was to evaluate a PPAAm nanolayer on zirconia after a storage period of 5 years. Zirconia specimens were directly coated with PPAAm (ZA0) or stored in aseptic packages at room temperature for 5 years (ZA5). Uncoated zirconia specimens (Zmt) and the micro-structured endosseous surface of a zirconia implant (Z14) served as controls. The elemental compositions of the PPAAm coatings were characterized and the viability, spreading and gene expression of human osteoblastic cells (MG-63) were assessed. The presence of amino groups in the PPAAm layer was significantly decreased after 5 years due to oxidation processes. Cell viability after 24 h was significantly higher on uncoated specimens (Zmt) than on all other surfaces. Cell spreading after 20 min was significantly higher for Zmt = ZA0 > ZA5 > Z14, while, after 24 h, spreading also varied significantly between Zmt > ZA0 > ZA5 > Z14. The expression of the mRNA differentiation markers collagen I and osteocalcin was upregulated on untreated surfaces Z14 and Zmt when compared to the PPAAm specimens. Due to the high biocompatibility of zirconia itself, a PPAAm coating may not additionally improve cell behavior.
钛的等离子体聚合烯丙胺（PPAAm）涂层可增强成骨细胞的细胞行为。本研究的目的是评估在储存5年后氧化锆上的PPAAm纳米层。将氧化锆标本直接用PPAAm涂层（ZA0）处理，或在室温下无菌包装中储存5年（ZA5）。未涂层的氧化锆标本（Zmt）和氧化锆种植体的微结构骨内表面（Z14）作为对照。对PPAAm涂层的元素组成进行了表征，并评估了人成骨细胞（MG-63）的活力、铺展情况和基因表达。由于氧化过程，5年后PPAAm层中氨基的含量显著降低。24小时后，未涂层标本（Zmt）上的细胞活力显著高于所有其他表面。20分钟后，Zmt = ZA0 > ZA5 > Z14的细胞铺展情况显著更高，而24小时后，Zmt > ZA0 > ZA5 > Z14之间的铺展情况也有显著差异。与PPAAm标本相比，未处理表面Z14和Zmt上的mRNA分化标志物I型胶原蛋白和骨钙素的表达上调。由于氧化锆本身具有高生物相容性，PPAAm涂层可能不会额外改善细胞行为。
