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绿豆分离蛋白/羧甲基魔芋葡甘露聚糖钠水凝胶的构建与表征:凝胶特性、结构特性、微观结构、钠盐释放及3D打印

Construction and characterisation of mung bean protein isolate/carboxymethyl konjac glucomannan sodium hydrogels: Gel properties, structural properties, microstructure, sodium salt release, and 3D printing.

作者信息

Zhang Kexin, Tan Zhifeng, Zhang Qingyi, Wu Qiong, Zhao Jiaxin, Xu Wensi, Liu Yuxin, Liu Xiaoyang, Zhou Dayong, Li Deyang

机构信息

SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, Liaoning Province Key Laboratory for Marine Food Science and Technology, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.

Liaoning General Fair Testing Co., Ltd., Shenyang 110000, China.

出版信息

Food Chem. 2025 Apr 30;472:142995. doi: 10.1016/j.foodchem.2025.142995. Epub 2025 Jan 20.

Abstract

This study proposed a hydrogel system using mung bean protein isolate (MPI) and carboxymethyl konjac glucomannan (CKGM). The effects of CKGM addition on the gel properties, structural characteristics, and Na loading capacity of the MPI-CKGM system were investigated. FTIR and molecular docking techniques demonstrated that MPI and CKGM formed hydrogels via hydrogen bonding interactions. The addition of CKGM led to the increase in gel strength of the hydrogel, reaching a peak value at a CKGM concentration of 3.5 %, which was attributed to the formation of a denser network structure. All hydrogels exhibited excellent performance in 3D printing applications. Fluorescence microscopy results demonstrated that the MPI-CKGM hydrogel was capable of loading Na. Upon increasing the CKGM concentration to 3.5 %, the Na loading rate increased from 83.03 % to 91.20 %, thereby increasing Na release rate. The findings of this study can provide a foundation for further understanding the salt reduction mechanisms of hydrogels.

摘要

本研究提出了一种使用绿豆分离蛋白(MPI)和羧甲基魔芋葡甘露聚糖(CKGM)的水凝胶体系。研究了添加CKGM对MPI-CKGM体系凝胶特性、结构特征和钠负载能力的影响。傅里叶变换红外光谱(FTIR)和分子对接技术表明,MPI和CKGM通过氢键相互作用形成水凝胶。添加CKGM导致水凝胶的凝胶强度增加,在CKGM浓度为3.5%时达到峰值,这归因于形成了更致密的网络结构。所有水凝胶在3D打印应用中均表现出优异性能。荧光显微镜结果表明,MPI-CKGM水凝胶能够负载钠。将CKGM浓度提高到3.5%时,钠负载率从83.03%提高到91.20%,从而提高了钠释放率。本研究结果可为进一步了解水凝胶的减盐机制提供基础。

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