Imanaka Takahiro, Takezawa Kentaro, Kujime Yuma, Kitakaze Hiroaki, Tsujimura Go, Kuribayashi Sohei, Sekii Yosuke, Ueda Norichika, Fukuhara Shinichiro, Kiuchi Hiroshi, Negoro Hiromitsu, Nonomura Norio
Department of Urology, Osaka University Graduate School of Medicine, 2-2, Yamadaoka, Suita-city, 565-0871, Osaka, Japan.
Glickman Urological & Kidney Institute, Cleveland Clinic Foundation, Cleveland, OH, USA.
Sci Rep. 2025 Jan 28;15(1):3589. doi: 10.1038/s41598-025-86526-6.
Nocturnal polyuria is a major cause of nocturia, which affects quality of life. Aging-related decreases in nitric oxide production have been reported to contribute to salt-induced nocturnal polyuria. We posited that enhanced nitric oxide production from exercise could mitigate salt-induced nocturnal polyuria. To validate, we assessed the impact of 4 weeks of moderate-intensity exercise on intrarenal renin-angiotensin system activity and salt-induced polyuria during inactive periods in 80-week-old mice. The moderate-intensity exercise group (Mod-Ex) had increased nitric oxide production and suppressed intrarenal renin-angiotensin system compared to the sedentary group (Sed) (renal angiotensinogen level: Sed vs. Mod-Ex, 1.101 ± 0.077 vs. 0.630 ± 0.048 arbitrary unit, P = 0.002). This exercise also alleviated salt-induced polyuria during inactive periods (diurnal polyuria index: Sed vs. Mod-Ex, 0.292 ± 0.027 vs. 0.193 ± 0.023, P = 0.018). These effects were neutralized by suppression of nitric oxide production by oral administration of N-Nitro-L-arginine methyl ester hydrochloride, an nitric oxide synthesis inhibitor. These findings indicate that exercise-induced improvements in salt-induced polyuria during inactive periods are caused by increases in nitric oxide production. In summary, increasing nitric oxide production may serve as a treatment method for salt-induced nocturnal polyuria in older adults.
夜间多尿是夜尿症的主要原因，会影响生活质量。据报道，与衰老相关的一氧化氮生成减少会导致盐诱导的夜间多尿。我们推测，运动增强一氧化氮生成可减轻盐诱导的夜间多尿。为进行验证，我们评估了4周中等强度运动对80周龄小鼠非活动期肾内肾素-血管紧张素系统活性和盐诱导多尿的影响。与久坐组（Sed）相比，中等强度运动组（Mod-Ex）一氧化氮生成增加，肾内肾素-血管紧张素系统受到抑制（肾血管紧张素原水平：Sed组与Mod-Ex组，1.101±0.077 vs. 0.630±0.048任意单位，P = 0.002）。该运动还减轻了非活动期盐诱导的多尿（日间多尿指数：Sed组与Mod-Ex组，0.292±0.027 vs. 0.193±0.023，P = 0.018）。口服一氧化氮合成抑制剂盐酸N-硝基-L-精氨酸甲酯抑制一氧化氮生成后，这些作用被抵消。这些发现表明，运动诱导的非活动期盐诱导多尿改善是由一氧化氮生成增加所致。总之，增加一氧化氮生成可能是治疗老年人盐诱导夜间多尿的一种方法。
