Tomonaga Taisuke, Izumi Hiroto, Nishida Chinatsu, Sato Kazuma, Nakamura Yuiko, Morimoto Toshiki, Higashi Yasuyuki, Wang Ke-Yong, Higashi Hidenori, Kojima Takuma, Sakurai Kazuo, Takeshita Jun-Ichi, Moriyama Akihiro, Yamasaki Kei, Yatera Kazuhiro, Morimoto Yasuo
Department of Occupational Pneumology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan.
Department of Environmental Health Engineering, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan.
Sci Rep. 2025 Jan 28;15(1):3514. doi: 10.1038/s41598-025-87174-6.
Polyacrylic acid (PAA) with different concentrations of cross-linker was instilled into the trachea of rats to examine the effect of PAA crosslink density on lung disorders. Methods: F344 rats were intratracheally exposed to low and high doses of PAA with cross-linker concentrations of 0.1, 1.0, and 5.0% (CL0.1%, CL1.0%, and CL5.0%, respectively). Rats were sacrificed at 3 days, 1 week, 1 month, 3 months, and 6 months after exposure. PAA with different cross-linker concentrations caused an increase in neutrophil influx, cytokine-induced neutrophils, and chemotactic factor (CINC) in bronchoalveolar lavage fluid (BALF) from 3 days to 1 week after instillation. Lactate dehydrogenase (LDH) activity in BALF and heme oxygenase-1 (HO-1) release in lung tissue were higher in the CL0.1% exposure group during the acute phase. Lung histopathological findings also showed that severe fibrotic changes induced by CL0.1% were greater than those observed in CL1.0% and CL5.0% exposure during the observation period. CL0.1% was associated with more severe lung fibrosis, and a decrease in lung fibrosis was observed with increasing cross-linker concentrations, suggesting that the cross-link density of PAA is a physicochemical feature that affects lung disorders.
将不同交联剂浓度的聚丙烯酸（PAA）滴入大鼠气管，以研究PAA交联密度对肺部疾病的影响。方法：将F344大鼠经气管内暴露于低剂量和高剂量的PAA，其交联剂浓度分别为0.1%、1.0%和5.0%（分别为CL0.1%、CL1.0%和CL5.0%）。在暴露后3天、1周、1个月、3个月和6个月处死大鼠。不同交联剂浓度的PAA在滴注后3天至1周内导致支气管肺泡灌洗液（BALF）中的中性粒细胞流入、细胞因子诱导的中性粒细胞和趋化因子（CINC）增加。急性期，CL0.1%暴露组BALF中的乳酸脱氢酶（LDH）活性和肺组织中的血红素加氧酶-1（HO-1）释放较高。肺组织病理学结果还显示，在观察期内，CL0.1%诱导的严重纤维化改变大于CL1.0%和CL5.0%暴露组。CL0.1%与更严重的肺纤维化相关，并且随着交联剂浓度的增加观察到肺纤维化减少，这表明PAA的交联密度是影响肺部疾病的一种物理化学特征。
