Burch W M, Corda G
Endocrinology. 1985 May;116(5):1724-8. doi: 10.1210/endo-116-5-1724.
To determine whether calcitonin (CT) might effect maturation of mammalian growth plate cartilage, we administered salmon CT (sCT) to young rats and used the growth plate from the distal metatarsal as our in vivo growth plate model. Growth plate alkaline phosphatase activity and histological examination were assessed after 3 days of sCT treatment. Alkaline phosphatase activity, a marker of hypertrophied chondrocytes, increased 85%, and the zone of maturation enlarged in rats receiving sCT. In addition, sCT treatment was associated with an increased growth plate 35SO4 incorporation 84% above animals receiving buffer alone. We tested whether this might be a direct effect of CT by using an in vitro model, the growth plate from the fetal pig scapula. Organ culture of these cartilages in serum-free medium with and without sCT (1 U/ml) was performed for 3 days. sCT stimulated alkaline phosphatase activity 49% above growth plates incubated in medium alone. Furthermore, sCT treatment increased the number of hypertrophied chondrocytes leading to widening of the zone of maturation. These studies suggest that CT might have a role growing mammals by promoting maturation of growth plate cartilage.
为了确定降钙素（CT）是否会影响哺乳动物生长板软骨的成熟，我们给幼鼠注射了鲑鱼降钙素（sCT），并使用远端跖骨的生长板作为我们的体内生长板模型。在sCT治疗3天后，评估生长板碱性磷酸酶活性和组织学检查。碱性磷酸酶活性是肥大软骨细胞的标志物，在接受sCT的大鼠中增加了85%，并且成熟区扩大。此外，与仅接受缓冲液的动物相比，sCT治疗使生长板35SO4掺入增加了84%。我们通过使用体外模型——胎猪肩胛骨的生长板，来测试这是否可能是CT的直接作用。将这些软骨在含和不含sCT（1 U/ml）的无血清培养基中进行器官培养3天。与仅在培养基中孵育的生长板相比，sCT刺激碱性磷酸酶活性增加了49%。此外，sCT治疗增加了肥大软骨细胞的数量，导致成熟区增宽。这些研究表明，CT可能通过促进生长板软骨的成熟在生长中的哺乳动物中发挥作用。
