Lyu Danni, Ni Shuang, Xu Jia, Zhu Sha, Xu Jing-Wei, Feng Yixuan, Shi Ce, Xu Wen
Eye Center of the 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou, Zhejiang, China.
Front Med (Lausanne). 2025 Jan 14;11:1537770. doi: 10.3389/fmed.2024.1537770. eCollection 2024.
To report a singular case of cataract caused by toad venom inoculation and to scrutinize the pathological mechanisms through proteomic sequencing of the lens specimen.
A young Chinese male presented with progressively deteriorating vision in his right eye subsequent to a history of toad venom inoculation. He was diagnosed with a toxic cataract, and underwent phacoemulsification cataract surgery. Anterior capsule, nucleus, and cortex specimens from the patient (designated as PT_CAP, PT_PHACO, and PT_CTX, respectively) and age-related cataract controls (C_CAP, C_PHACO, and C_CTX, respectively) were collected and subjected to 4D label-free quantitative proteomics.
A multitude of differentially expressed proteins (DEPs) were identified in the patient's lens compared to those in the controls. Specifically, a total of 204 DEPs were identified in PT_CAP compared to C_CAP, with MYH6, MYL2, MYL3, STAT1, and ANK1 among the foremost regulated DEPs. The DEPs of PT_CAP were principally affiliated with functions including "transportation of small molecules," "regulation of metal ion transport," and "import into cell." A sum of 109 DEPs were delineated in PT_CTX compared to C_CTX, with TPM1 among the top-10 downregulated DEPs. Ninety-five DEPs were pinpointed in PT_PHACO compared to C_PHACO, with hexokinase among the top 10 downregulated DEPs. These proteins were ascertained to be linked with Na/K-ATPase activity.
This study introduced the first documented case of toxic cataract caused by toad venom inoculation. Proteomic sequencing indicated a correlation between cataract and alterations in Na/K-ATPase activity, providing insights for the clinical management of ocular toad venom inoculation in subsequent cases.
报告一例因蟾蜍毒液接种导致白内障的罕见病例，并通过晶状体标本的蛋白质组测序研究其病理机制。
一名中国年轻男性在有蟾蜍毒液接种史后，右眼视力逐渐下降。他被诊断为中毒性白内障，并接受了超声乳化白内障手术。收集了患者的前囊、核和皮质标本（分别命名为PT_CAP、PT_PHACO和PT_CTX）以及年龄相关性白内障对照组标本（分别命名为C_CAP、C_PHACO和C_CTX），并进行了4D无标记定量蛋白质组学分析。
与对照组相比，在患者的晶状体中鉴定出大量差异表达蛋白（DEP）。具体而言，与C_CAP相比，PT_CAP中总共鉴定出204个DEP，其中MYH6、MYL2、MYL3、STAT1和ANK1是调控最显著的DEP。PT_CAP的DEP主要与“小分子运输”、“金属离子运输调节”和“细胞内导入”等功能相关。与C_CTX相比，PT_CTX中划定了109个DEP，其中TPM1是下调最明显的前10个DEP之一。与C_PHACO相比，PT_PHACO中确定了95个DEP，其中己糖激酶是下调最明显的前10个DEP之一。这些蛋白质被确定与钠钾ATP酶活性有关。
本研究介绍了首例有记录的因蟾蜍毒液接种导致的中毒性白内障病例。蛋白质组测序表明白内障与钠钾ATP酶活性改变之间存在关联，为后续眼部蟾蜍毒液接种病例的临床管理提供了见解。
