Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A&F University, Hangzhou 311300, China.
Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass, College of Chemistry and Materials Engineering, Zhejiang A&F University, Hangzhou 311300, China.
Molecules. 2023 Apr 21;28(8):3628. doi: 10.3390/molecules28083628.
Toad venom is a traditional Chinese medicine with high medicinal value. The existing quality evaluation standards of toad venom have obvious limitations because of the lack of research on proteins. Thus, it is necessary to screen suitable quality markers and establish appropriate quality evaluation methods for toad venom proteins to guarantee their safety and efficacy in clinical applications. SDS-PAGE, HPLC, and cytotoxicity assays were used to analyze differences in protein components of toad venom from different areas. Functional proteins were screened as potential quality markers by proteomic and bioinformatic analyses. The protein components and small molecular components of toad venom were not correlated in content. Additionally, the protein component had strong cytotoxicity. Proteomics analysis showed that 13 antimicrobial proteins, four anti-inflammatory and analgesic proteins, and 20 antitumor proteins were differentially expressed extracellular proteins. A candidate list of functional proteins was coded as potential quality markers. Moreover, Lysozyme C-1, which has antimicrobial activity, and Neuropeptide B (NPB), which has anti-inflammatory and analgesic activity, were identified as potential quality markers for toad venom proteins. Quality markers can be used as the basis of quality studies of toad venom proteins and help to construct and improve safe, scientific, and comprehensive quality evaluation methods.
蟾酥是一种具有高药用价值的中药。由于对蛋白质的研究不足，现有的蟾酥质量评价标准存在明显的局限性。因此，有必要筛选合适的质量标志物，并建立适当的蟾酥蛋白质质量评价方法，以保证其在临床应用中的安全性和有效性。采用 SDS-PAGE、HPLC 和细胞毒性试验分析了不同地区蟾酥中蛋白质成分的差异。通过蛋白质组学和生物信息学分析筛选功能蛋白作为潜在的质量标志物。蟾酥的蛋白质成分和小分子成分在含量上没有相关性。此外，蛋白质成分具有很强的细胞毒性。蛋白质组学分析表明，蟾酥细胞外差异表达的蛋白质中含有 13 种抗菌蛋白、4 种抗炎和镇痛蛋白和 20 种抗肿瘤蛋白。候选功能蛋白列表被编码为潜在的质量标志物。此外，具有抗菌活性的溶菌酶 C-1 和具有抗炎和镇痛活性的神经肽 B（NPB）被鉴定为蟾酥蛋白质的潜在质量标志物。质量标志物可用作蟾酥蛋白质质量研究的基础，有助于构建和完善安全、科学、全面的质量评价方法。
