• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

hsa-miR-548d-3p：一种靶向黄病毒科多个成员核衣壳和/或衣壳基因的潜在微小RNA。

hsa-miR-548d-3p: a potential microRNA to target nucleocapsid and/or capsid genes in multiple members of the Flaviviridae family.

作者信息

Cayatineto H W, Hakim S T

机构信息

Hakim's Lab, Department of Biology, School of STEM, Diné College, Tuba City, AZ, United States.

出版信息

Front Bioinform. 2025 Jan 14;4:1487292. doi: 10.3389/fbinf.2024.1487292. eCollection 2024.

DOI:10.3389/fbinf.2024.1487292
PMID:39877236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11772435/
Abstract

INTRODUCTION

Flaviviridae comprise a group of enveloped, positive-stranded RNA viruses that are mainly transmitted through either mosquitoes or tick bites and/or contaminated blood, blood products, or other body secretions. These viruses cause diseases ranging from mild to severe and are considered important human pathogens. MicroRNAs (miRNAs) are non-coding molecules involved in growth, development, cell proliferation, protein synthesis, apoptosis, and pathogenesis. These small molecules are even being used as gene suppressors in antiviral therapeutics, inhibiting viral replication. In the current study, we used bioinformatic tools to predict a possible miRNA sequence that could be complementary to the nucleocapsid (NP) and/or capsid (CP) gene of the Flaviviridae family and provide an inhibitory solution.

METHODS

Bioinformatics is a field of science that includes tremendous computational analysis, logarithms, and sequence alignments. To predict the right alignments between miRNA and viral mRNA genomes, we used computational databases such as miRBase, NCBI, and Basic Alignment Search Tool-nucleotides (BLAST-n).

RESULTS

Of the 2,600 mature miRNAs, hsa-miR-548d-3p revealed complementary sequences with the flavivirus capsid gene and bovine viral diarrhea virus (BVDV) capsid gene and was selected as a possible candidate to inhibit flaviviruses.

CONCLUSION

Although more detailed and studies are required to test the possible inhibitory effects of hsa-miR-548d-3p against flaviviruses, this computational study may be the first step to study further, developing a novel therapeutic for lethal viruses within the Flaviviridae family using suggested candidate miRNAs.

摘要

引言

黄病毒科包含一组包膜的正链RNA病毒，主要通过蚊虫叮咬或蜱叮咬和/或受污染的血液、血液制品或其他身体分泌物传播。这些病毒可引起从轻度到重度的疾病，被认为是重要的人类病原体。微小RNA（miRNA）是参与生长、发育、细胞增殖、蛋白质合成、细胞凋亡和发病机制的非编码分子。这些小分子甚至被用作抗病毒治疗中的基因抑制剂，抑制病毒复制。在本研究中，我们使用生物信息学工具预测了一种可能与黄病毒科核衣壳（NP）和/或衣壳（CP）基因互补的miRNA序列，并提供了一种抑制解决方案。

方法

生物信息学是一个科学领域，包括大量的计算分析、对数和序列比对。为了预测miRNA与病毒mRNA基因组之间的正确比对，我们使用了诸如miRBase、NCBI和基本比对搜索工具-核苷酸（BLAST-n）等计算数据库。

结果

在2600个成熟miRNA中，hsa-miR-548d-3p显示出与黄病毒衣壳基因和牛病毒性腹泻病毒（BVDV）衣壳基因的互补序列，并被选为抑制黄病毒的可能候选物。

结论

尽管需要更详细的研究来测试hsa-miR-548d-3p对黄病毒的可能抑制作用，但这项计算研究可能是进一步研究的第一步，利用建议的候选miRNA开发针对黄病毒科致死病毒的新型疗法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/af14f6ab32d9/fbinf-04-1487292-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/2e1d7c9cda94/fbinf-04-1487292-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/b541d5007e08/fbinf-04-1487292-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/02c4abf87700/fbinf-04-1487292-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/512ce5b2a9e4/fbinf-04-1487292-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/7f88ed6374bc/fbinf-04-1487292-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/c9fcfe96922a/fbinf-04-1487292-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/d5b988447d63/fbinf-04-1487292-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/b2fb06ff6152/fbinf-04-1487292-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/636206b419e5/fbinf-04-1487292-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/1f272a4b5e8e/fbinf-04-1487292-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/0f1f4fabb75c/fbinf-04-1487292-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/34a4131d47b2/fbinf-04-1487292-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/2b402f5ef239/fbinf-04-1487292-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/3daa589cb70c/fbinf-04-1487292-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/ddfa9bfe5ec2/fbinf-04-1487292-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/af14f6ab32d9/fbinf-04-1487292-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/2e1d7c9cda94/fbinf-04-1487292-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/b541d5007e08/fbinf-04-1487292-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/02c4abf87700/fbinf-04-1487292-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/512ce5b2a9e4/fbinf-04-1487292-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/7f88ed6374bc/fbinf-04-1487292-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/c9fcfe96922a/fbinf-04-1487292-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/d5b988447d63/fbinf-04-1487292-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/b2fb06ff6152/fbinf-04-1487292-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/636206b419e5/fbinf-04-1487292-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/1f272a4b5e8e/fbinf-04-1487292-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/0f1f4fabb75c/fbinf-04-1487292-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/34a4131d47b2/fbinf-04-1487292-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/2b402f5ef239/fbinf-04-1487292-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/3daa589cb70c/fbinf-04-1487292-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/ddfa9bfe5ec2/fbinf-04-1487292-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/493c/11772435/af14f6ab32d9/fbinf-04-1487292-g016.jpg

相似文献

1
hsa-miR-548d-3p: a potential microRNA to target nucleocapsid and/or capsid genes in multiple members of the Flaviviridae family.hsa-miR-548d-3p：一种靶向黄病毒科多个成员核衣壳和/或衣壳基因的潜在微小RNA。
Front Bioinform. 2025 Jan 14;4:1487292. doi: 10.3389/fbinf.2024.1487292. eCollection 2024.
2
A MicroRNA Screen Identifies the Wnt Signaling Pathway as a Regulator of the Interferon Response during Flavivirus Infection.一项微小RNA筛选确定Wnt信号通路是黄病毒感染期间干扰素反应的调节因子。
J Virol. 2017 Mar 29;91(8). doi: 10.1128/JVI.02388-16. Print 2017 Apr 15.
3
SARS-COV-2 as potential microRNA sponge in COVID-19 patients.SARS-CoV-2 作为 COVID-19 患者中的潜在 miRNA 海绵。
BMC Med Genomics. 2022 Apr 23;15(Suppl 2):94. doi: 10.1186/s12920-022-01243-7.
4
Predicting microRNAs and their Target Genes Involved in Sepsis Pathogenesis by using Bioinformatics Methods.运用生物信息学方法预测参与脓毒症发病机制的微小RNA及其靶基因。
Curr Pharm Des. 2025;31(13):1067-1077. doi: 10.2174/0113816128304401241031094647.
5
Triplex-forming MicroRNAs form stable complexes with HIV-1 provirus and inhibit its replication.形成三链体的微小RNA与HIV-1前病毒形成稳定复合物并抑制其复制。
Appl Immunohistochem Mol Morphol. 2010 Dec;18(6):532-45. doi: 10.1097/PAI.0b013e3181e1ef6a.
6
Identification of plasma miR-4505, miR-4743-5p and miR-4750-3p as novel diagnostic biomarkers for coronary artery disease in patients with type 2 diabetes mellitus: a case-control study.鉴定血浆 miR-4505、miR-4743-5p 和 miR-4750-3p 作为 2 型糖尿病患者冠心病的新型诊断生物标志物：病例对照研究。
Cardiovasc Diabetol. 2024 Jul 29;23(1):278. doi: 10.1186/s12933-024-02374-0.
7
MicroRNAs in oral fluids (saliva and gingival crevicular fluid) as biomarkers in orthodontics: systematic review and integrated bioinformatic analysis.口腔液（唾液和龈沟液）中的 microRNAs 作为正畸学中的生物标志物：系统评价和综合生物信息学分析。
Prog Orthod. 2021 Oct 11;22(1):31. doi: 10.1186/s40510-021-00377-1.
8
Identification of Hub Genes Associated with Hypertension and Their Interaction with miRNA Based on Weighted Gene Coexpression Network Analysis (WGCNA) Analysis.基于加权基因共表达网络分析（WGCNA）的高血压相关枢纽基因鉴定及其与 miRNA 的相互作用。
Med Sci Monit. 2020 Sep 5;26:e923514. doi: 10.12659/MSM.923514.
9
Deciphering inhibitory mechanism of coronavirus replication through host miRNAs-RNA-dependent RNA polymerase interactome.通过宿主微小RNA-依赖RNA的RNA聚合酶相互作用组解析冠状病毒复制的抑制机制。
Front Genet. 2022 Aug 26;13:973252. doi: 10.3389/fgene.2022.973252. eCollection 2022.
10
Differential microRNA expression in the peripheral blood from human patients with COVID-19.COVID-19 患者外周血中的差异 microRNA 表达。
J Clin Lab Anal. 2020 Oct;34(10):e23590. doi: 10.1002/jcla.23590. Epub 2020 Sep 22.

本文引用的文献

1
Insights into Online microRNA Bioinformatics Tools.在线微小RNA生物信息学工具的见解
Noncoding RNA. 2023 Mar 6;9(2):18. doi: 10.3390/ncrna9020018.
2
Quercetin Inhibits Hsp70 Blocking of Bovine Viral Diarrhea Virus Infection and Replication in the Early Stage of Virus Infection.槲皮素在牛病毒性腹泻病毒感染早期抑制 HSP70 阻断感染和复制。
Viruses. 2022 Oct 26;14(11):2365. doi: 10.3390/v14112365.
3
Transfusion-transmitted arboviruses: Update and systematic review.经输血传播的虫媒病毒：最新进展和系统评价。
PLoS Negl Trop Dis. 2022 Oct 6;16(10):e0010843. doi: 10.1371/journal.pntd.0010843. eCollection 2022 Oct.
4
The NS4A Protein of Classical Swine Fever Virus Suppresses RNA Silencing in Mammalian Cells.经典猪瘟病毒 NS4A 蛋白抑制哺乳动物细胞中的 RNA 沉默。
J Virol. 2022 Aug 10;96(15):e0187421. doi: 10.1128/jvi.01874-21. Epub 2022 Jul 13.
5
Non-structural proteins of bovine viral diarrhea virus.牛病毒性腹泻病毒的非结构蛋白。
Virus Genes. 2022 Dec;58(6):491-500. doi: 10.1007/s11262-022-01914-8. Epub 2022 May 25.
6
miR-548d-3p Is Up-Regulated in Human Visceral Leishmaniasis and Suppresses Parasite Growth in Macrophages.miR-548d-3p 在人类内脏利什曼病中上调，并抑制巨噬细胞中的寄生虫生长。
Front Cell Infect Microbiol. 2022 Feb 10;12:826039. doi: 10.3389/fcimb.2022.826039. eCollection 2022.
7
Pathogenesis and virulence of flavivirus infections.黄病毒感染的发病机制和毒力。
Virulence. 2021 Dec;12(1):2814-2838. doi: 10.1080/21505594.2021.1996059.
8
Hsa-mir-548 family expression in human reproductive tissues.人生殖组织中 hsa-mir-548 家族的表达。
BMC Genom Data. 2021 Oct 8;22(1):40. doi: 10.1186/s12863-021-00997-w.
9
The key amino acids of E protein involved in early flavivirus infection: viral entry.参与早期黄病毒感染的 E 蛋白关键氨基酸：病毒进入。
Virol J. 2021 Jul 3;18(1):136. doi: 10.1186/s12985-021-01611-2.
10
miR-548d-3p Alters Parasite Growth and Inflammation in Infection.miR-548d-3p 改变 感染中的寄生虫生长和炎症反应。
Front Cell Infect Microbiol. 2021 Jun 10;11:687647. doi: 10.3389/fcimb.2021.687647. eCollection 2021.