Olson Jenessa, Pennance Tom, Spaan Johannie M, Odiere Maurice R, Tennessen Jacob A, Steinauer Michelle L
Western University of Health Sciences, 200 Mullins Dr, Lebanon, OR, 97355, USA.
Centre for Global Health Research, Kenya Medical Research Institute (KEMRI), P. O. Box 1578-40100, Kisumu, Kenya.
Curr Res Parasitol Vector Borne Dis. 2025 Jan 6;7:100241. doi: 10.1016/j.crpvbd.2025.100241. eCollection 2025.
snails are intermediate hosts for schistosome parasites, which cause morbidity and mortality in humans worldwide. We aimed to determine the mating system of , a hermaphroditic vector of schistosomiasis in the African Great Lakes, with the goal of informing the design of genetic studies such as linkage mapping to improve genome assembly and genetic association studies to identify snail resistance genes. To determine the relative rates of outcrossing selfing, we assayed the progeny of experimental crosses of snails in the laboratory using a PCR and restriction enzyme digest to determine snail genotype and parentage. Out of 7 experimental crosses and 56 total offspring assayed, 100% were derived from outcrossing rather than inbreeding. These results indicate that is primarily an outcrossing species, although previous work has shown that this species retains the capability of self-fertilization.
蜗牛是血吸虫寄生虫的中间宿主，这种寄生虫在全球范围内导致人类发病和死亡。我们旨在确定非洲大湖地区血吸虫病雌雄同体传播媒介的交配系统，目的是为基因研究（如连锁图谱绘制以改进基因组组装以及基因关联研究以识别蜗牛抗性基因）的设计提供信息。为了确定异交与自交的相对比率，我们在实验室中对蜗牛的实验杂交后代进行了检测，使用聚合酶链反应（PCR）和限制性内切酶消化来确定蜗牛的基因型和亲子关系。在检测的7次实验杂交和总共56个后代中，100%来自异交而非近亲繁殖。这些结果表明，尽管之前的研究表明该物种保留了自我受精的能力，但它主要是一个异交物种。
