Alshahrani Saeed, Ashafaq Mohammad, Jali Abdulmajeed M, Almoshari Yosif, Alam Mohammad Intakhab, Al Shahi Hamad, Alshamrani Ayed A, Hussain Sohail
Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, 45142, Jazan, Saudi Arabia.
Department of Pharmaceutics, College of Pharmacy, Jazan University, 45142, Jazan, Saudi Arabia.
Naunyn Schmiedebergs Arch Pharmacol. 2025 Jan 29. doi: 10.1007/s00210-025-03825-5.
Cypermethrin is a pyrethroid showing nephrotoxicity by generating ROS-impaired oxidative stress and changes in inflammatory and apoptotic markers. The harmful consequences are intended to be mitigated by the imbalance between oxidants and antioxidants. The anti-inflammatory and antioxidant possessions of nanocurcumin (NC) with improved bioavailability ameliorate Cyp toxicity in rat kidneys. In our study, Group I was the control while Group II was treated alone with NC (5 mg). Group III was given 50 mg/kg of Cyp for two weeks. Groups IV, V, and VI received Cyp as in group III and co-treatment with varying NC doses after 5 days of Cyp dosing, respectively. All treatments were given orally for two weeks. After the termination of the study, LPO, 4-HNE, GSH, antioxidant catalase, and SOD were evaluated as markers of inflammation and apoptosis along with ELISA, qRT-PCR, and histopathology were used to assess morphological changes. Our work has shown that Cyp causes nephrotoxicity by altering all parameters. The Cyp-treated group was shown to have higher expression of the oxidative stress marker LPO and inflammatory interleukins as well as Bax, NF-kB, caspase-3, and caspase-9. Although LPO, inflammation, and apoptosis are reduced, antioxidant status is improved by NC.
氯氰菊酯是一种拟除虫菊酯，通过产生活性氧介导的氧化应激以及炎症和凋亡标志物的变化而表现出肾毒性。氧化剂和抗氧化剂之间的失衡旨在减轻这些有害后果。具有改善生物利用度的纳米姜黄素（NC）的抗炎和抗氧化特性可改善大鼠肾脏中的氯氰菊酯毒性。在我们的研究中，第一组为对照组，第二组单独用NC（5毫克）治疗。第三组给予50毫克/千克的氯氰菊酯，持续两周。第四组、第五组和第六组在给予氯氰菊酯5天后分别接受与第三组相同剂量的氯氰菊酯，并联合不同剂量的NC进行治疗。所有治疗均口服两周。研究结束后，评估脂质过氧化（LPO）、4-羟基壬烯醛（4-HNE）、谷胱甘肽（GSH）、抗氧化酶过氧化氢酶和超氧化物歧化酶（SOD）作为炎症和凋亡的标志物，同时使用酶联免疫吸附测定（ELISA）、定量逆转录聚合酶链反应（qRT-PCR）和组织病理学来评估形态学变化。我们的研究表明，氯氰菊酯通过改变所有参数而导致肾毒性。氯氰菊酯治疗组显示氧化应激标志物LPO、炎症白细胞介素以及Bax、核因子κB（NF-κB）、半胱天冬酶-3和半胱天冬酶-9的表达较高。虽然脂质过氧化、炎症和凋亡有所减少，但纳米姜黄素改善了抗氧化状态。
