Lee Su-Min, Seo Eunsu, Kim Yang-Hoon, Min Jiho
Graduate School of Semiconductor and Chemical Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-Gu, Jeonju, Jeonbuk 54896, South Korea.
School of Biological Sciences, Chungbuk National University, 1 Chungdae-Ro, Seowon-Gu, Cheongju 28644, South Korea; Biomedical Research Institute, Chungbuk National University Hospital, 1 Sunhwan-Ro, Seowon-Gu, Cheongju 28644, South Korea.
Biochim Biophys Acta Mol Cell Res. 2025 Mar;1872(3):119907. doi: 10.1016/j.bbamcr.2025.119907. Epub 2025 Jan 27.
Senescence significantly contributes to aging in various tissues, influenced by factors such as lysosomal alkalinization, which disrupts autophagic flux and accumulates toxic substances. This disruption leads to oxidative stress, increased lysosomal permeability, cellular senescence, and apoptosis. Similar to mammalian lysosomes, S. cerevisiae-derived vacuoles degrade macromolecules using hydrolytic enzymes and mitigate these aging effects. Our study assessed the anti-aging potential of yeast vacuoles in human lung fibroblasts treated with hydrogen peroxide (HO). Pretreatment with vacuoles at concentrations of 1, 5, and 10 μg/ml decreased SA-β-gal-positive cell counts, reduced mRNA levels of senescence markers (p21 and p53), and senescence-associated secretory phenotype (SASP) factors (IL-6 and TNF-α) compared to controls treated with HO alone. Additionally, these vacuoles significantly diminished intracellular reactive oxygen species (ROS) levels, indicating their potential as effective lung anti-senescence agents. This study suggests that yeast vacuoles could be used as a preventive measure against changes associated with lung aging.
细胞衰老在多种组织的老化过程中起着重要作用，它受到溶酶体碱化等因素的影响，溶酶体碱化会破坏自噬流并积累有毒物质。这种破坏会导致氧化应激、溶酶体通透性增加、细胞衰老和凋亡。与哺乳动物的溶酶体类似，酿酒酵母来源的液泡利用水解酶降解大分子，并减轻这些衰老效应。我们的研究评估了酵母液泡对用过氧化氢（HO）处理的人肺成纤维细胞的抗衰老潜力。与仅用HO处理的对照组相比，用浓度为1、5和10μg/ml的液泡预处理可减少SA-β-半乳糖苷酶阳性细胞计数，降低衰老标志物（p21和p53）和衰老相关分泌表型（SASP）因子（IL-6和TNF-α）的mRNA水平。此外，这些液泡显著降低了细胞内活性氧（ROS）水平，表明它们具有作为有效的肺部抗衰老剂的潜力。这项研究表明，酵母液泡可作为预防与肺部衰老相关变化的措施。
