Identification and characteristics of differentially expressed genes under UV-B stress in .

OBJECTIVE

This study aimed to screen the differentially expressed genes (DEGs) of under UV-B stress and identify the significant pathways based on gene enrichment analysis results.

METHODS

In this study, the allotetraploid crop was used to examine changes in various physiological indexes under UV-B stress, and screened out all DEGs under UV-B stress (16 kJ m d) based on six leaf transcriptomes. The main enrichment pathways of DEGs were analyzed according to gene annotation. Finally, we predicted the regulatory genes of phenylpropanoid pathway under UV-B stress by co-expression network analysis, and selected for verification.

RESULTS

Gene annotation analysis revealed that DEGs were predominantly enriched in pathways related to photosynthesis and secondary metabolism. Further analysis revealed that UV-B stress impaired photosynthesis mainly by damaging photosystem II (PSII) and inhibiting electron transport, whereas responded to UV-B stress by synthesizing secondary metabolites such as anthocyanins and lignin. We selected the regulatory genes for verification. It was found to be an anthocyanin negative regulator in response to UV-B stress.

CONCLUSIONS

UV-B stress impaired photosynthesis mainly by damaging photosystem II (PSII) and inhibiting electron transport, whereas responded to UV-B stress by synthesizing secondary metabolites such as anthocyanins and lignin.