Price G B, Benzing K, Stewart S, Grover J
Immunol Lett. 1985;9(1):9-14. doi: 10.1016/0165-2478(85)90086-0.
An IgG monoclonal antibody that detects a subpopulation of lymphocytes found in peripheral blood and bone marrow of patients with CLL and malignant lymphoma is described. The initial immunization used to achieve the resultant monoclonal antibody included the use of cells obtained by DNA transformation of mouse L-cells with the DNA obtained from a morphologically altered somatic cell hybrid between primary human CLL peripheral lymphocytes and a flat-revertant Chinese hamster ovary (CHO) cell line designated GRC+L-73. Hybridomas were thus selected as potentially recognizing antigens associated with the morphological transformation induced by hybridization of CHO cells with lymphocytes from lymphocytic malignancies. One such hybridoma, designated 37-28, was selected for further investigation. The monoclonal antibody produced was IgG (gamma G2a) and detects a subpopulation of lymphocytes present in hematological specimens of some of the lymphocytic malignancies.
描述了一种IgG单克隆抗体，它可检测慢性淋巴细胞白血病（CLL）和恶性淋巴瘤患者外周血及骨髓中的一个淋巴细胞亚群。用于获得所得单克隆抗体的初始免疫包括使用通过用从原发性人类CLL外周淋巴细胞与一种指定为GRC + L - 73的扁平回复中国仓鼠卵巢（CHO）细胞系之间形态改变的体细胞杂种获得的DNA对小鼠L细胞进行DNA转化而获得的细胞。因此，选择杂交瘤作为可能识别与CHO细胞与淋巴细胞恶性肿瘤中的淋巴细胞杂交诱导的形态转化相关的抗原。选择了一种这样的杂交瘤，命名为37 - 28，进行进一步研究。产生的单克隆抗体为IgG（γG2a），可检测一些淋巴细胞恶性肿瘤血液学标本中存在的淋巴细胞亚群。
