Price G B, Sturgeon J F, Till J E
Blood. 1980 Feb;55(2):351-4.
We have examined the properties of hybrid cells formed by polyethylene glycol-mediated fusion of the GRC+L-73 line of Chinese hamster ovary (CHO) cells with peripheral blood cells from patients with chronic lymphocytic leukemia (CLL) or with bone marrow cells from patients with malignant lymphoma. The results indicate that hybrid cells can be detected by their ability to form "foci" of characteristic morphology in the presence of a monolayer of parental CHO cells and that clones isolated from such foci express aspects of the differentiation status, as detected by immunologic markers, of the human parental cells.
我们已经检测了通过聚乙二醇介导，将中国仓鼠卵巢（CHO）细胞的GRC + L - 73系与慢性淋巴细胞白血病（CLL）患者的外周血细胞或恶性淋巴瘤患者的骨髓细胞融合而形成的杂交细胞的特性。结果表明，在亲本CHO细胞单层存在的情况下，杂交细胞可通过其形成特征形态“集落”的能力来检测，并且从这些集落中分离出的克隆表达了人类亲本细胞通过免疫标记检测到的分化状态的某些方面。
