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通过过表达 TaLAX1 提高小麦的再生和遗传转化效率。

Enhancing wheat regeneration and genetic transformation through overexpression of TaLAX1.

机构信息

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China.

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China.

出版信息

Plant Commun. 2024 May 13;5(5):100738. doi: 10.1016/j.xplc.2023.100738. Epub 2023 Oct 28.

DOI:10.1016/j.xplc.2023.100738
PMID:37897039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11121199/
Abstract

In the realm of genetically transformed crops, the process of plant regeneration holds utmost significance. However, the low regeneration efficiency of several wheat varieties currently restricts the use of genetic transformation for gene functional analysis and improved crop production. This research explores overexpression of TaLAX PANICLE1 (TaLAX1), which markedly enhances regeneration efficiency, thereby boosting genetic transformation and genome editing in wheat. Particularly noteworthy is the substantial increase in regeneration efficiency of common wheat varieties previously regarded as recalcitrant to genetic transformation. Our study shows that increased expression of TaGROWTH-REGULATING FACTOR (TaGRF) genes, alongside that of their co-factor, TaGRF-INTERACTING FACTOR 1 (TaGIF1), enhances cytokinin accumulation and auxin response, which may play pivotal roles in the improved regeneration and transformation of TaLAX1-overexpressing wheat plants. Overexpression of TaLAX1 homologs also significantly increases the regeneration efficiency of maize and soybean, suggesting that both monocot and dicot crops can benefit from this enhancement. Our findings shed light on a gene that enhances wheat genetic transformation and elucidate molecular mechanisms that potentially underlie wheat regeneration.

摘要

在转基因作物领域，植物再生过程具有至关重要的意义。然而，目前几种小麦品种的再生效率较低，限制了遗传转化在基因功能分析和改良作物生产中的应用。本研究探索了 TaLAX PANICLE1（TaLAX1）的过表达，它显著提高了再生效率，从而促进了小麦的遗传转化和基因组编辑。特别值得注意的是，以前被认为难以进行遗传转化的普通小麦品种的再生效率有了大幅提高。我们的研究表明，TaGROWTH-REGULATING FACTOR（TaGRF）基因及其共因子 TaGRF-INTERACTING FACTOR 1（TaGIF1）的表达增加，增强了细胞分裂素的积累和生长素的响应，这可能在 TaLAX1 过表达小麦植株的再生和转化改良中发挥关键作用。TaLAX1 同源物的过表达也显著提高了玉米和大豆的再生效率，这表明单子叶和双子叶作物都可以受益于这种增强。我们的研究结果揭示了一个增强小麦遗传转化的基因，并阐明了潜在的小麦再生的分子机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/f35c41c5f24f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/52f82f2d9312/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/5538ed045a54/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/69d649cb6362/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/e01aaf184c20/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/73ebad24dd5b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/c1bfa901d11d/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/f35c41c5f24f/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/52f82f2d9312/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/5538ed045a54/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/69d649cb6362/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/e01aaf184c20/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/73ebad24dd5b/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/c1bfa901d11d/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31df/11121199/f35c41c5f24f/gr7.jpg

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