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利用辅助分型技术进行空肠弯曲菌和结肠弯曲菌感染的流行病学研究。

Use of auxotyping for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections.

作者信息

Tenover F C, Knapp J S, Patton C, Plorde J J

出版信息

Infect Immun. 1985 May;48(2):384-8. doi: 10.1128/iai.48.2.384-388.1985.

Abstract

A chemically defined medium developed for Neisseria gonorrhoeae was modified to support the growth of Campylobacter jejuni and Campylobacter coli. A total of 76 isolates of C. jejuni and 14 isolates of C. coli were tested on this medium, which was designated Campylobacter defined medium (CDM), over a 3-month period. Although none of the C. coli isolates appeared to require amino acids, 51% of the C. jejuni tested required one and 7% required multiple amino acids for growth. An analysis of isolates obtained from three household outbreaks of campylobacteriosis demonstrated that auxotyping identified the epidemic strain within each outbreak. Among 70 isolates of C. jejuni examined, no correlation could be drawn between a specific serotype and auxotype or between auxotype and plasmid profile.

摘要

一种为淋病奈瑟菌开发的化学成分明确的培养基经过改良,以支持空肠弯曲菌和结肠弯曲菌的生长。在3个月的时间里,总共76株空肠弯曲菌分离株和14株结肠弯曲菌分离株在这种被命名为空肠弯曲菌限定培养基(CDM)的培养基上进行了测试。虽然所有结肠弯曲菌分离株似乎都不需要氨基酸,但所测试的空肠弯曲菌中有51%需要一种氨基酸才能生长,7%需要多种氨基酸才能生长。对从三次家庭弯曲菌病暴发中获得的分离株进行的分析表明,营养分型确定了每次暴发中的流行菌株。在检测的70株空肠弯曲菌分离株中,无法得出特定血清型与营养型之间或营养型与质粒图谱之间的相关性。

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