Lior H, Woodward D L, Edgar J A, Laroche L J, Gill P
J Clin Microbiol. 1982 May;15(5):761-8. doi: 10.1128/jcm.15.5.761-768.1982.
A serotyping scheme for Campylobacter jejuni was developed based on slide agglutination of live bacteria with whole cell antisera absorbed with homologous heated and heterologous unheated cross-reactive antigens. Among 815 isolates from human and nonhuman sources, 21 serogroups were recognized. Of the 615 isolates from human cases of gastroenteritis, 529 (86%) were typable; 455 strains agglutinated in 20 single antisera, whereas 74 isolates agglutinated in various pairs of antisera, allowing subdivision of some main serogroups into subserogroups. Of the 200 isolates of C. jejuni from nonhuman sources (chicken, swine, etc.), 166 (83%) were typable, 145 cultures agglutinated in various single antisera, and 21 strains agglutinated with different pairs of antisera. Among isolates from all sources, 8 serogroups (1, 2, 4, 5, 7, 8, 9, and 11) were encountered most frequently. Serogroups 1, 2, 4, 5, 7, 9, and 11 were most common among human isolates; the majority of the chicken and all of the swine isolates belonged to the same serogroups identified from human cases. Very good serological correlation was obtained in 20 family outbreaks and 4 community outbreaks.
基于活菌与经同源加热和异源未加热交叉反应抗原吸收的全细胞抗血清进行玻片凝集反应，开发了一种空肠弯曲菌血清分型方案。在815株来自人类和非人类来源的分离株中，识别出21个血清群。在615株来自人类胃肠炎病例的分离株中，529株（86%）可分型；455株在20种单一抗血清中凝集，而74株在各种抗血清对中凝集，这使得一些主要血清群可细分为亚血清群。在200株来自非人类来源（鸡、猪等）的空肠弯曲菌分离株中，166株（83%）可分型，145株培养物在各种单一抗血清中凝集，21株与不同的抗血清对凝集。在所有来源的分离株中，8个血清群（1、2、4、5、7、8、9和11）最为常见。血清群1、2、4、5、7、9和11在人类分离株中最为常见；大多数鸡和所有猪的分离株属于从人类病例中鉴定出的相同血清群。在20起家庭暴发和4起社区暴发中获得了很好的血清学相关性。
