Hébert G A, Hollis D G, Weaver R E, Lambert M A, Blaser M J, Moss C W
J Clin Microbiol. 1982 Jun;15(6):1065-73. doi: 10.1128/jcm.15.6.1065-1073.1982.
Several biochemical test systems were studied for their potential usefulness for the examination of strains of Campylobacter species. Most (81%) of the C. jejuni strains hydrolyzed sodium hippurate, but strains of C. fetus, C. sputorum, and C. fecalis did not. Some (46%) of the C. jejuni strains and all of the C. sputorum subsp. sputorum, C. sputorum subsp. bubulus, and C. fecalis strains hydrolyzed DNA, but the C. fetus and C. sputorum subsp. mucosalis strains did not. Strains of all species of Campylobacter grew on charcoal-yeast extract agar, but 47% of the C. jejuni strains did not. Alkaline phosphatase activity was recorded for some strains of C. jejuni, but all other species were negative for this activity. Aryl sulfatase activity was detected in 7% of the C. jejuni, 15% of the C. fetus subsp. fetus, and all of the C. sputorum subsp. sputorum, C. sputorum subsp. bubulus, and C. fecalis strains, but it was not detected in the C. fetus subsp. venerealis and C. sputorum subsp. mucosalis strains. Most (93%) of the C. jejuni but none of the other Campylobacter strains contained lactobacillic acid when examined for cellular fatty acids. On the basis of results from three of these tests (hippurate hydrolysis, DNA hydrolysis, and growth on charcoal-yeast extract agar), clinical strains of C. jejuni were placed in eight biotypes.
研究了几种生化检测系统对弯曲杆菌属菌株检测的潜在用途。大多数（81%）空肠弯曲杆菌菌株能水解马尿酸钠，但胎儿弯曲杆菌、唾液弯曲杆菌和粪便弯曲杆菌菌株不能。一些（46%）空肠弯曲杆菌菌株以及所有唾液弯曲杆菌亚种唾液弯曲杆菌、唾液弯曲杆菌亚种泡状弯曲杆菌和粪便弯曲杆菌菌株能水解DNA，但胎儿弯曲杆菌和唾液弯曲杆菌亚种黏膜弯曲杆菌菌株不能。所有弯曲杆菌属菌种的菌株都能在活性炭酵母提取物琼脂上生长，但47%的空肠弯曲杆菌菌株不能。一些空肠弯曲杆菌菌株有碱性磷酸酶活性，但所有其他菌种的该活性均为阴性。在7%的空肠弯曲杆菌、15%的胎儿弯曲杆菌亚种胎儿弯曲杆菌以及所有唾液弯曲杆菌亚种唾液弯曲杆菌、唾液弯曲杆菌亚种泡状弯曲杆菌和粪便弯曲杆菌菌株中检测到芳基硫酸酯酶活性，但在胎儿弯曲杆菌亚种性病弯曲杆菌和唾液弯曲杆菌亚种黏膜弯曲杆菌菌株中未检测到。在检测细胞脂肪酸时，大多数（93%）空肠弯曲杆菌菌株含有乳杆菌酸，而其他弯曲杆菌菌株均不含。根据其中三项检测（马尿酸水解、DNA水解和在活性炭酵母提取物琼脂上生长）的结果，将空肠弯曲杆菌临床菌株分为八个生物型。
