Hasan Md Rajib, Raju Md Rasel, Lim Un Taek
Department of Plant Medicals, Andong National University, Andong, Republic of Korea.
PLoS One. 2025 Jan 30;20(1):e0317483. doi: 10.1371/journal.pone.0317483. eCollection 2025.
Beauveria bassiana AAD16, isolated from Allomyrina dichotoma, shows promise as a mycoinsecticide against various coleopterans. However, assessing non-target impacts on beneficial beetles like ladybirds is crucial before commercialization. Here we assessed the compatibility between ladybird beetles and B. bassiana AAD16. The virulence of the AAD16 strain was compared with that of an available commercial strain, B. bassiana GHA, for two developmental stages (adults and 3rd instar larvae) of two coccinellids, Harmonia axyridis Pallas and Chilocorus spp. Say using the topical (1μl) application method. The ST50 for the two life stages of the two ladybird beetles were not different between the two tested fungal strains. Mycosis rates recorded from the dead bodies were also not significant except in the 3rd instar which showed 36 and 63% from AAD16 and GHA strains in H. axyridis, while those of Chilocorus spp. were 40 and 63%, respectively. In adult stage, the mycosis rates of H. axyridis (males and females tested separately) were (20-23) % and (26-30) % from the AAD16 and GHA strains, while those of Chilocorus spp. (unsexed) were 23 and 30%, respectively. AAD16 caused similar rates of mortality in the adult stages of both species. Therefore, we conclude that B. bassiana AAD16 would not increase risk to these beneficial insects compared to a similar pathogen commercialized.
从独角仙中分离得到的球孢白僵菌AAD16，有望成为一种防治多种鞘翅目昆虫的真菌杀虫剂。然而，在商业化之前，评估其对七星瓢虫等有益甲虫的非靶标影响至关重要。在此，我们评估了七星瓢虫与球孢白僵菌AAD16之间的兼容性。采用点滴（1μl）接种法，比较了AAD16菌株与市售菌株球孢白僵菌GHA对两种瓢虫（异色瓢虫和食螨瓢虫）两个发育阶段（成虫和3龄幼虫）的毒力。在两种受试真菌菌株之间，两种瓢虫两个生命阶段的半数致死时间没有差异。除3龄幼虫外，从死亡虫体记录的真菌病发生率也无显著差异，在异色瓢虫中，AAD16和GHA菌株的真菌病发生率分别为36%和63%，而食螨瓢虫的分别为40%和63%。在成虫阶段，AAD16和GHA菌株对异色瓢虫（分别对雄性和雌性进行测试）的真菌病发生率分别为（20 - 23）%和（26 - 30）%，而对食螨瓢虫（未区分性别）的分别为23%和30%。AAD16在两种瓢虫成虫阶段引起的死亡率相似。因此，我们得出结论，与已商业化的类似病原体相比，球孢白僵菌AAD16不会增加对这些有益昆虫的风险。
