Vivekanandhan Perumal, Swathy Kannan, Alahmadi Tahani Awad, Ansari Mohammad Javed
Department of General Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, India.
Society for Research and Initiatives for Sustainable Technologies and Institutions, Ahmedabad, Gujarat, India.
Front Microbiol. 2024 Feb 12;15:1336334. doi: 10.3389/fmicb.2024.1336334. eCollection 2024.
In this study, we conducted tests on the isolation, identification, characterization, and extraction of chemical molecules from against larvae. The enzyme responses of to the crude extract were examined 24 h after treatment, and the number of dead larvae was calculated 24 and 48 h after treatment. Molecular docking studies were conducted to assess the interaction of important molecules with the acetylcholinesterase enzyme. The larvicidal activity of crude chemicals from fungi was high 24 h after treatment, with LC and LC values of 25.937 and 33.559 μg/mL, respectively. For a period of 48 h, the LC and LC values were 52.254 and 60.450 μg/mL, respectively. The levels of acetylcholinesterase, α-carboxylesterase, and β-carboxylesterase enzymes were lower in the treatment group after 24 h compared to the control group. The GC-MS test revealed that the crude extract consisted mainly of 9,10-octadecadienoic acid, which was the primary compound. Docking results indicated that 9,10-octadecadienoic acid showed a strong interaction with acetylcholinesterase (AChE). Our findings suggest that the chemical molecule 9,10-octadecadienoic acid derived from the entomopathogenic fungus is more toxic to larvae. We plan to conduct studies to test its effectiveness in semi-field conditions and to evaluate its stability in field conditions. We believe that this 9,10-octadecadienoic acid molecule could be used to control larvae in the near future without causing environmental pollution.
在本研究中，我们针对从[某种真菌]中分离、鉴定、表征及提取化学分子以对抗[某种害虫]幼虫开展了试验。处理24小时后检测了[某种害虫]对粗提物的酶反应，并在处理24小时和48小时后计算死亡幼虫的数量。进行了分子对接研究以评估重要分子与乙酰胆碱酯酶的相互作用。真菌粗化学物质的杀幼虫活性在处理24小时后较高，LC50和LC90值分别为25.937和33.559μg/mL。在48小时内，LC50和LC90值分别为52.254和60.450μg/mL。与对照组相比，处理组在24小时后乙酰胆碱酯酶、α - 羧酸酯酶和β - 羧酸酯酶的水平较低。气相色谱 - 质谱联用（GC - MS）测试表明粗提物主要由9,10 - 十八碳二烯酸组成，这是主要化合物。对接结果表明9,10 - 十八碳二烯酸与乙酰胆碱酯酶（AChE）有强烈的相互作用。我们的研究结果表明，源自昆虫病原真菌[某种真菌]的化学分子9,10 - 十八碳二烯酸对[某种害虫]幼虫毒性更强。我们计划开展研究以测试其在半田间条件下的有效性，并评估其在田间条件下的稳定性。我们相信这种9,10 - 十八碳二烯酸分子在不久的将来可用于控制[某种害虫]幼虫，且不会造成环境污染。
