Krause Sandra T, Frey Maximilian
Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle, Germany.
Department of Cell and Metabolic Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
Methods Mol Biol. 2025;2895:73-82. doi: 10.1007/978-1-0716-4350-1_6.
The extraction of plant essential oils (EOs) and analysis by gas chromatography coupled to mass spectrometry (GC-MS) are standard methods when studying aromatic plants and the chemical composition of EOs. Here, two simple methods for the extraction of EO compounds from leaves of Thymus vulgaris are described. Organic solvent extraction and solid-phase microextraction (SPME), respectively, are used and the results of the GC-MS analyses are compared. We explain how analyte peaks can be identified and discuss possible rearrangement reactions that can occur during the extraction process or analysis.
在研究芳香植物及其精油的化学成分时，提取植物精油（EOs）并通过气相色谱-质谱联用（GC-MS）进行分析是标准方法。本文描述了两种从百里香叶中提取EO化合物的简单方法。分别采用有机溶剂萃取和固相微萃取（SPME），并比较了GC-MS分析结果。我们解释了如何识别分析物峰，并讨论了在提取过程或分析过程中可能发生的重排反应。
