Wang Qiang, Sohrabi Reza, Tholl Dorothea
Department of Biological Sciences, Virginia Tech, 220 Ag Quad Lane, Blacksburg, VA, 24061, USA.
Methods Mol Biol. 2014;1153:149-59. doi: 10.1007/978-1-4939-0606-2_10.
Terpene specialized metabolites exhibit multiple functions in plant-environment interactions and plant development. Molecular biologists investigating the biochemistry and molecular function of terpenes need to apply robust but yet sensitive analytical methods optimized and adapted to the structural diversity and often varying concentrations of terpene compounds in plant tissues. Here we present hands-on protocols for sample preparation and GC-MS or LC-MS/MS analysis of selected diterpene and triterpene hydrocarbons or oxygenated derivatives from roots and shoots of Arabidopsis and rice.
萜类特殊代谢产物在植物与环境的相互作用以及植物发育过程中发挥着多种功能。研究萜类生物化学和分子功能的分子生物学家需要应用强大且灵敏的分析方法，这些方法要经过优化并适应植物组织中萜类化合物的结构多样性以及常常变化的浓度。在此，我们提供了实际操作方案，用于拟南芥和水稻根与地上部分中选定的二萜和三萜碳氢化合物或氧化衍生物的样品制备以及气相色谱 - 质谱联用（GC-MS）或液相色谱 - 串联质谱联用（LC-MS/MS）分析。
