Abdel-Nasser Aya, Fathy Hayam M, Badr Ahmed N, Barakat Olfat S, Hathout Amal S
Food Toxicology and Contaminants Department, National Research Centre, Egypt.
Agricultural Microbiology Department, Faculty of Agriculture, Cairo University, Egypt.
Heliyon. 2025 Jan 10;11(2):e41875. doi: 10.1016/j.heliyon.2025.e41875. eCollection 2025 Jan 30.
is a severe danger to worldwide maize () cultivation, due to its extreme toxicity of aflatoxins produced by the fungi, and its ability to cause economic losses while also posing a health concern to humans and animals. Among the measures that may be considered for control, applying coatings based on natural ingredients appears to be the most promising. The current work examines the antagonistic ability of bioactive metabolites added to chitosan nanoparticles against on maize kernels. The chitosan nanoparticles loaded with bioactive metabolites were characterized using the transmission electron microscope (TEM), zeta potential, size distribution, polydiversity index (PDI), pH, encapsulation efficiency and Fourier transform infrared spectroscopy (FTIR). The TEM revealed that the chitosan nanoparticles loaded with bioactive metabolites were spherical and smooth on the surface, and by increasing the concentration of bioactive metabolites added to the chitosan nanoparticles, the diameter of the chitosan nanoparticle grew. The zeta potential and size distribution values increased as the quantity of bioactive metabolites increased in the chitosan nanoparticles. The FTIR analysis indicated the presence of several functional groups, including alkynes, alkene, aliphatic primary amines, and other functional groups. The chitosan nanoparticles loaded with bioactive metabolites at a concentration of 7 mg/mL showed significant antifungal activity against , reducing their growth in maize kernels by 89.42 % after 10 days of storage. They also reduced the percentage of germination inhibition rate and viability percentage. It could be concluded that adding bioactive metabolites to chitosan nanoparticles might have significant implications for food safety by using it in the industry to reduce the fungal contamination of grains.
由于真菌产生的黄曲霉毒素具有极高的毒性，且它能够造成经济损失，同时还对人类和动物的健康构成威胁，因此对全球玉米种植来说是一种严重的危害。在可考虑用于控制的措施中，应用基于天然成分的涂层似乎是最有前景的。当前的研究考察了添加到壳聚糖纳米颗粒中的生物活性代谢产物对玉米籽粒上的的拮抗能力。使用透射电子显微镜（TEM）、zeta电位、尺寸分布、多分散指数（PDI）、pH值、包封率和傅里叶变换红外光谱（FTIR）对负载生物活性代谢产物的壳聚糖纳米颗粒进行了表征。TEM显示，负载生物活性代谢产物的壳聚糖纳米颗粒呈球形且表面光滑，并且随着添加到壳聚糖纳米颗粒中的生物活性代谢产物浓度的增加，壳聚糖纳米颗粒的直径增大。随着壳聚糖纳米颗粒中生物活性代谢产物数量的增加，zeta电位和尺寸分布值也增加。FTIR分析表明存在几个官能团，包括炔烃、烯烃、脂肪族伯胺和其他官能团。浓度为7mg/mL的负载生物活性代谢产物的壳聚糖纳米颗粒对显示出显著的抗真菌活性，在储存10天后，其在玉米籽粒中的生长减少了89.42%。它们还降低了发芽抑制率和活力百分比。可以得出结论，将生物活性代谢产物添加到壳聚糖纳米颗粒中，通过在工业中使用它来减少谷物的真菌污染，可能对食品安全具有重要意义。
