Nanobiology and Phytotherapy Laboratory, Department of Botany, University of North Bengal, Darjeeling, WB, 734013, India.
ANMOL Laboratory, Department of Biotechnology, University of North Bengal, Darjeeling, WB, 734013, India.
Sci Rep. 2022 Aug 25;12(1):14518. doi: 10.1038/s41598-022-18670-2.
Agro-researchers are endlessly trying to derive a potential biomolecule having antifungal properties in order to replace the application of synthetic fungicides on agricultural fields. Rot disease often caused by Fusarium solani made severe loss of wheat crops every year. Chitosan and its metallic nano-derivatives hold a broad-spectrum antifungal property. Our interdisciplinary study deals with the application of nickel chitosan nanoconjugate (NiCNC) against Fusarium rot of wheat, in comparison with chitosan nanoparticles (CNPs) and commercial fungicide Mancozeb. CNPs and NiCNC were characterized on the basis of UV-Vis spectrophotometry, HR-TEM, FESEM, EDXS and FT-IR. Both CNPs and NiCNC were found effective against the fungal growth, of which NiCNC at 0.04 mg/mL showed complete termination of F. solani grown in suitable medium. Ultrastructural analysis of F. solani conidia treated with NiCNC revealed pronounced damages and disruption of the membrane surface. Fluorescence microscopic study revealed generation of oxidative stress in the fungal system upon NiCNC exposure. Moreover, NiCNC showed reduction in rot disease incidence by 83.33% of wheat seedlings which was further confirmed through the observation of anatomical sections of the stem. NiCNC application helps the seedling to overcome the adverse effect of pathogen, which was evaluated through stress indices attributes.
农业研究人员一直在努力提取具有抗真菌特性的潜在生物分子，以替代在农业领域应用合成杀菌剂。镰刀菌引起的根腐病每年都会给小麦作物造成严重损失。壳聚糖及其金属纳米衍生物具有广谱的抗真菌特性。我们的跨学科研究涉及应用镍壳聚糖纳米复合物（NiCNC）来防治小麦镰刀菌腐烂，与壳聚糖纳米颗粒（CNPs）和商业杀菌剂代森锰锌进行比较。根据紫外可见分光光度法、高分辨率透射电子显微镜、场发射扫描电子显微镜、能谱和傅里叶变换红外光谱对 CNPs 和 NiCNC 进行了表征。CNPs 和 NiCNC 都被发现对真菌生长有效，其中 NiCNC 在 0.04mg/mL 时显示出完全终止在适宜培养基中生长的 F. solani。用 NiCNC 处理的 F. solani 分生孢子的超微结构分析显示出明显的膜表面损伤和破坏。荧光显微镜研究显示，NiCNC 暴露会在真菌系统中产生氧化应激。此外，NiCNC 使小麦幼苗根腐病的发病率降低了 83.33%，通过观察茎的解剖切片进一步证实了这一点。NiCNC 的应用有助于幼苗克服病原菌的不利影响，这可以通过应激指标属性来评估。
