Jashnsaz Hossein, Neuert Gregor
Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, Nashville, TN 37232, USA.
Department of Pharmacology, School of Medicine, Vanderbilt University, Nashville, TN 37232, USA.
iScience. 2024 Dec 19;28(1):111625. doi: 10.1016/j.isci.2024.111625. eCollection 2025 Jan 17.
How cells respond to dynamic environmental changes is crucial for understanding fundamental biological processes and cell physiology. In this study, we developed an experimental and quantitative analytical framework to explore how dynamic stress gradients that change over time regulate cellular volume, signaling activation, and growth phenotypes. Our findings reveal that gradual stress conditions substantially enhance cell growth compared to conventional acute stress. This growth advantage correlates with a minimal reduction in cell volume dependent on the dynamic of stress. We explain the growth phenotype with our finding of a logarithmic signal transduction mechanism in the yeast mitogen-activated protein kinase (MAPK) osmotic stress response pathway. These insights into the interplay between gradual environments, cell volume change, dynamic cell signaling, and growth, advance our understanding of fundamental cellular processes in gradual stress environments.
细胞如何应对动态环境变化对于理解基本生物学过程和细胞生理学至关重要。在本研究中，我们开发了一个实验性和定量分析框架，以探索随时间变化的动态应力梯度如何调节细胞体积、信号激活和生长表型。我们的研究结果表明，与传统的急性应激相比，逐渐施加的应激条件显著促进细胞生长。这种生长优势与依赖于应激动态的细胞体积最小减少相关。我们通过在酵母丝裂原活化蛋白激酶（MAPK）渗透应激反应途径中发现对数信号转导机制来解释生长表型。这些对逐渐变化的环境、细胞体积变化、动态细胞信号传导和生长之间相互作用的见解，推进了我们对逐渐应激环境中基本细胞过程的理解。
