Chen Yuanfang, Huang Yiqian, Yang Yuhe R
CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, CAS, Beijing 100190, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
JACS Au. 2024 Dec 27;5(1):17-27. doi: 10.1021/jacsau.4c00986. eCollection 2025 Jan 27.
DNA nanostructures present new opportunities as Nanotags for electron microscopy (EM) imaging, leveraging their high programmability, unique shapes, biomolecule conjugation capability, and stability compatible with standard cryogenic sample preparation protocols. This perspective highlights the potential of DNA Nanotags to enable high-throughput multiplexed EM analysis and facilitate particle identification for cryogenic electron tomography (cryo-ET). Meanwhile, applying Nanotags in live-cell environments requires the efficient cellular uptake of intact structures and successful cytosolic migration. Promising strategies such as employing direct cytosolic delivery platforms and expressing RNA-based Nanotags are discussed, while more systematic studies are needed to fully understand the intracellular trafficking and achieve precise localization of DNA Nanotags.
DNA纳米结构作为用于电子显微镜（EM）成像的纳米标签展现了新的机遇，这得益于其高度的可编程性、独特的形状、生物分子共轭能力以及与标准低温样品制备方案兼容的稳定性。这一观点突出了DNA纳米标签在实现高通量多重EM分析以及促进低温电子断层扫描（cryo-ET）的颗粒识别方面的潜力。与此同时，在活细胞环境中应用纳米标签需要完整结构有效地被细胞摄取并成功迁移至胞质。文中讨论了诸如采用直接胞质递送平台和表达基于RNA的纳米标签等有前景的策略，不过还需要更系统的研究来全面了解细胞内运输情况并实现DNA纳米标签的精确定位。
