Liu Kun, Qin Yonglin, Wu Lan, Yi Rong, Shi Xiaohua, Yu Jing, Shi Xiaohong, Dong Wenzhuo, Jia Liguo, Fan Mingshou
College of Agronomy, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Front Plant Sci. 2025 Jan 16;15:1487794. doi: 10.3389/fpls.2024.1487794. eCollection 2024.
The HAK/KUP/KT (High-affinity K transporters/K uptake permeases/K transporters) is the largest and most dominant potassium transporter family in plants, playing a crucial role in various biological processes. However, our understanding of HAK/KUP/KT gene family in potato ( L.) remains limited and unclear. In this study, 24 genes () were identified through a genome-wide analysis and were found to be unevenly distributed across ten chromosomes. Based on phylogenetic analysis, these gene family members were classified into four distinct clusters. All StHAK protein sequences contained the conserved motifs and domains. Promoter cis-acting elements analysis revealed that most gene family members in potatoes were associated with responses to light and hormones such as abscisic acid or methyl jasmonate, however, many motifs responsive to hormones and stress conditions have not been clearly studied or reported in plants. Synteny analysis suggested that 33, 19, 8, 1 genes were orthologous to those in soybean, cassava, Arabidopsis and rice, respectively. The previously published RNA-seq results, transcriptomic data and qRT-PCR experiments indicated that the expression profiles of these genes were tissue-specific and were influenced by multiple factors, including biotic and abiotic stress, hormone, potassium fertilizer. To provide a clear and convenient view of gene expression across different tissues in potato, we generated a cartoon heatmap to vividly illustrate the tissue-specific expression of genes, which is unprecedented in the gene family analysis of potato. At last, we identified genes such as , , and with high expression in potato tubers using qRT-PCR, suggesting their potential involvement in tuber growth and development. This can contribute to a deeper understanding of the mechanism of potassium absorption and transportation in potatoes. It has laid a solid theoretical foundation for the genetic regulation of potassium nutritional efficiency in potatoes and the breeding of potato varieties with high potassium efficiency.
HAK/KUP/KT（高亲和钾转运体/钾吸收通透酶/钾转运体）是植物中最大且最主要的钾转运体家族，在各种生物学过程中发挥着关键作用。然而，我们对马铃薯（Solanum tuberosum L.）中HAK/KUP/KT基因家族的了解仍然有限且不清晰。在本研究中，通过全基因组分析鉴定出了24个StHAK基因，发现它们不均匀地分布在十条染色体上。基于系统发育分析，这些StHAK基因家族成员被分为四个不同的簇。所有StHAK蛋白序列都包含保守的基序和结构域。启动子顺式作用元件分析表明，马铃薯中大多数StHAK基因家族成员与对光和脱落酸或茉莉酸甲酯等激素的反应相关，然而，许多对激素和胁迫条件有反应的基序在植物中尚未得到明确研究或报道。共线性分析表明，分别有33个、19个、8个、1个基因与大豆、木薯、拟南芥和水稻中的基因直系同源。先前发表的RNA-seq结果、转录组数据和qRT-PCR实验表明，这些StHAK基因的表达谱具有组织特异性，并受到多种因素的影响，包括生物和非生物胁迫、激素、钾肥。为了清晰方便地展示马铃薯不同组织中StHAK基因的表达情况，我们生成了一个卡通热图来生动地说明StHAK基因的组织特异性表达，这在马铃薯基因家族分析中是前所未有的。最后，我们使用qRT-PCR鉴定出了如StHAK1、StHAK5和StHAK10等在马铃薯块茎中高表达的基因，表明它们可能参与块茎的生长和发育。这有助于更深入地了解马铃薯中钾吸收和运输的机制。它为马铃薯钾营养效率的遗传调控和高钾效率马铃薯品种的培育奠定了坚实的理论基础。
