Jobe A, Jacobs H, Ikegami M, Berry D
J Appl Physiol (1985). 1985 Apr;58(4):1246-51. doi: 10.1152/jappl.1985.58.4.1246.
To study the protein permeability properties of the ventilated premature lung, we delivered groups of eight lambs at 122 and 135 days gestational age and ventilated the lambs equivalently. The lambs at 122 days gestational age had been treated with natural sheep surfactant at birth, and both groups of lambs had similar pH and blood gas values to 3 h of age. Three groups of lambs at 146 days gestational age also were studied for comparison; four lambs were ventilated to normalized PCO2 values, four lambs were ventilated equivalently to the premature lambs with supplemental CO2 used to normalize PCO2 values, and four lambs were treated with natural surfactant and ventilated similarly to the preterm lambs. The percent recovery into an alveolar wash and lung tissue of 131I-albumin given by intravascular injection and of 125I-albumin given into the airways was measured in each animal after killing at 3 h of age. Full-term lambs had a small bidirectional leak of albumin to and from the alveoli and lung tissue. The recovery of intravascular 131I-albumin in the alveolar wash was 5.8- and 4.1-fold higher in lambs at 122 and 135 days gestational age, respectively, than in full-term lambs. The loss of 125I-albumin from the airways and alveoli also increased as gestational age decreased. The bidirectional flux of albumin to and from the alveoli increased as gestational age decreased in the prematurely delivered and ventilated lambs.
为研究通气早产肺的蛋白质通透性特性，我们选取了妊娠122天和135天的每组8只羔羊，对其进行同等通气。妊娠122天的羔羊出生时接受了天然羊肺表面活性物质治疗，两组羔羊在3小时龄时的pH值和血气值相似。还研究了三组妊娠146天的羔羊作为对照；4只羔羊通气至正常PCO2值，4只羔羊与早产羔羊同等通气，使用补充CO2使PCO2值正常化，4只羔羊接受天然表面活性物质治疗并与早产羔羊相似地通气。在3小时龄处死每只动物后，测量经血管注射给予的131I-白蛋白以及经气道给予的125I-白蛋白在肺泡灌洗物和肺组织中的回收百分比。足月羔羊白蛋白在肺泡与肺组织之间有少量双向渗漏。妊娠122天和135天的羔羊肺泡灌洗物中血管内131I-白蛋白的回收率分别比足月羔羊高5.8倍和4.1倍。随着胎龄降低，气道和肺泡中125I-白蛋白的损失也增加。在早产且通气的羔羊中，随着胎龄降低，白蛋白在肺泡与肺组织之间的双向通量增加。
