Nawaz Noor Ul Ain, Saeed Muhammad, Faizi Shaheen, Ali Irfan, Shafiq Muhammad, Shahid Muhammad, Rabbi Fazle, Khan Fahim Ullah, Sharif Rabeea, Ul-Haq Zaheer, Khan Khalid Mohammed, Hemeg Hassan A, Al Abdulmonem Waleed, Aljohani Abdullah S M, Rauf Abdur
Department of Pharmacy, City University of Science and Information Technology, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Department of Pharmacy, Qurtuba University of Science and Information Technology, Peshawar, Khyber Pakhtunkhwa, Pakistan.
Curr Top Med Chem. 2025;25(8):943-954. doi: 10.2174/0115680266333237241212071912.
In continuation of our chemical and biological work on , we evaluated the antipyretic activity of its extract which on fractionation gives a pure alkaloid galegine. Galegine a bioprivileged compound, is a hemiterpene bearing a guanidine group, which holds significant importance in medicinal chemistry. Biological activities such as antimicrobial, antidiabetic, anti-inflammatory, cardiovascular, anticancer, and antihypertensive, are often associated with guanidine-containing molecules.
Given the biological importance of guanidine and in search of safe antipyretic agents from natural resources, an antipyretic activity of methanolic extract of and galegine was conducted to discover a potential hypothermic drug candidate from the plant.
, the antipyretic activity of galegine (5, 25, and 50 mg/kg doses) and methanolic extract of (50, 100, and 200 mg/kg doses) was investigated by employing yeastinduced pyrexia in mice model. molecular docking analysis involving target enzymes cyclooxygenase- 1 (COX-1), cyclooxygenase-2 (COX-2), and microsomal prostaglandin E synthase-1 (mPGES-1) was conducted. Additionally, galegine underwent ADME/T profiling using SwissADME and Protox-II tools to evaluate its bioavailability and safety profiles.
Both the extract and galegine showed a progressive dose-dependent reduction in body temperatures of the hyperthermic test animals. Moreover, molecular docking analysis revealed significant binding affinities ranging from -3.58 to -5.41 kcal/mol. ADME/T analyses of galegine predicted its high drug-likeness and good safety profile.
These biological and computational approaches supported in addressing pyrexia, with the isolated compound galegine emerging as a promising antipyretic agent.
在我们对[具体物质]的化学和生物学研究的延续中，我们评估了其提取物的解热活性，该提取物经分馏得到一种纯生物碱胍丁胺。胍丁胺是一种具有生物活性的化合物，是一种带有胍基的半萜，在药物化学中具有重要意义。抗菌、抗糖尿病、抗炎、心血管、抗癌和抗高血压等生物活性通常与含胍分子有关。
鉴于胍的生物学重要性，并为从自然资源中寻找安全的解热剂，对[具体植物]的甲醇提取物和胍丁胺的解热活性进行了研究，以从该植物中发现一种潜在的降温药物候选物。
采用酵母诱导小鼠发热模型，研究了胍丁胺（5、25和50mg/kg剂量）和[具体植物]甲醇提取物（50、100和200mg/kg剂量）的解热活性。进行了涉及靶酶环氧化酶-1（COX-1）、环氧化酶-2（COX-2）和微粒体前列腺素E合酶-1（mPGES-1）的分子对接分析。此外，使用SwissADME和Protox-II工具对胍丁胺进行了ADME/T分析，以评估其生物利用度和安全性。
提取物和胍丁胺均使发热试验动物的体温呈剂量依赖性逐渐降低。此外，分子对接分析显示结合亲和力显著，范围为-3.58至-5.41kcal/mol。胍丁胺的ADME/T分析预测其具有高药物相似性和良好的安全性。
这些生物学和计算方法有助于解决发热问题，分离出的化合物胍丁胺有望成为一种解热剂。
