Álvarez-Rodríguez Manuel, García-Calvo Laura, Gardela Jaume, Ruiz-Conca Mateo, Olvera-Maneu Sergi, Martín-San Juan Adrián, López-Béjar Manel, Martinez-Pastor Felipe, Vicente-Carrillo Alejandro
Department of Animal Reproduction, INIA-CSIC, Madrid, Spain.
Department of Animal Health and Anatomy, Faculty of Veterinary Medicine, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Vet Med Sci. 2025 Mar;11(2):e70236. doi: 10.1002/vms3.70236.
Blood serum (BS) and seminal plasma (SP) share a plethora of compounds that might present an individual and/or temporal concentration variation. We aimed to determine whether BS and SP concentrations of albumin, calcium, citrate, creatinine, fructose, glucose, lactate, total protein, urea, zinc, cortisol, anti-Müllerian hormone (AMH) and testosterone are related to weekly collections in New Zealand White (NZW) adult rabbit bucks. During a 12-week study, blood samples were obtained at the beginning and the end of the study period, and semen samples were taken twice a week from four NZW adult rabbit bucks, starting at 6-7 months of age. After semen collection, the sperm motility was subjectively assessed, and SP was obtained by centrifugation. BS and SP were evaluated for the above-mentioned metabolites using a Biosystems BA400 automated analyser with commercial-specific kits or enzyme immunoassay (EIA) kits, assessing the effects of the male and time of collection. In addition, a correlation analysis aimed at disclosing associations between parameters in BS and SP was performed. Male effect was not significant for BS, but it was significant for SP albumin, citrate, fructose, glucose, lactate and total protein. In addition, all the correlations in BS were positive, whereas they were more balanced in SP, being close to half of the correlations. In conclusion, variations of some metabolites (albumin, citrate, fructose, glucose, lactate and total protein) appear to be potential biomarkers for rabbit SP, although further studies should test their usefulness for sperm fertility assessment.
血清（BS）和精浆（SP）含有大量可能存在个体和/或时间浓度变化的化合物。我们旨在确定新西兰白兔（NZW）成年公兔血清和精浆中白蛋白、钙、柠檬酸盐、肌酐、果糖、葡萄糖、乳酸、总蛋白、尿素、锌、皮质醇、抗苗勒管激素（AMH）和睾酮的浓度是否与每周采集情况相关。在一项为期12周的研究中，在研究期开始和结束时采集血样，从4只6 - 7月龄的NZW成年公兔开始，每周采集两次精液样本。采集精液后，主观评估精子活力，并通过离心获得精浆。使用配备商业专用试剂盒或酶免疫分析（EIA）试剂盒的Biosystems BA400自动分析仪评估血清和精浆中的上述代谢物，评估雄性和采集时间的影响。此外，进行了相关分析以揭示血清和精浆中参数之间的关联。雄性对血清的影响不显著，但对精浆中的白蛋白、柠檬酸盐、果糖、葡萄糖、乳酸和总蛋白有显著影响。此外，血清中的所有相关性均为正，而精浆中的相关性更为平衡，接近一半的相关性为正。总之，一些代谢物（白蛋白、柠檬酸盐、果糖、葡萄糖、乳酸和总蛋白）的变化似乎是兔精浆的潜在生物标志物，尽管进一步的研究应测试它们在评估精子生育能力方面的实用性。
