Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, P.O. Box 21531, Alexandria, Egypt.
Sci Rep. 2023 Aug 10;13(1):12987. doi: 10.1038/s41598-023-39928-3.
The harmful influences of global warming on rabbit reproduction and industry attract global attention. Zinc (Zn) is an important trace element with a wide list of functions in the male reproductive system. The aim of this study was to estimate the effects of different forms of zinc supplementation, as organic (Zn methionine), nano (nano Zn oxide) as indirect way to minimized it impact on environment, and inorganic (Zn sulphate) on physiological parameters, semen quality, anti-oxidative status, hormonal profiles of male rabbits subjected to server heat stress. Thirty-six V-line bucks (6-7 months old, 2842.29 ± 34.46 g weight) were randomly distributed to 4 groups, bucks in 1st group (control group, Con) fed basal diet without Zn supplementation, the 2nd, 3rd and 4th groups fed basial diet with 30 mg/kg of zinc methionine (Zn-Met), nano zinc oxide (ZnO-NPs), and zinc sulphate (Zn-S), respectively for a period of 10 weeks suffered from severe heat stress of environmental ambient temperature (over 32 of temperature humidity index, THI). Semen samples were collected and evaluated for volume, pH, motility, concentration, viability, initial fructose, and seminal plasma antioxidant concentration. Liver, kidney function, hormonal and antioxidant profile were measured in blood serum. The results revealed that, compared to control, all forms of Zn supplementation used significantly improved kidney function (creatinine), serum antioxidant (SOD and CAT), physiological parameters, especially on 1st month of the experiment, and seminal plasma antioxidant (SOD and CAT) of heat stressed bucks. Likewise, semen quality in terms of sperm concentration, sperm viability, and initial fructose enhanced significantly (P ≤ 0.05) by ZnO-NPs supplementation. Zinc methionine supplementation significantly improved liver function and decreased seminal plasma TBARs. Treatments with Zn-Met and ZnO-NPs increased seminal TAC and blood testosterone levels with reduced blood cortisol levels compared to other groups. Severe heat stress could be counteract by inclusion Zn with studied forms Zn-Met and ZnO-NPs at recommended dose (30 mg/kg diet) to improve semen quality and antioxidant status.
全球变暖对兔子繁殖和产业的有害影响引起了全球关注。锌（Zn）是一种重要的微量元素，在男性生殖系统中具有广泛的功能。本研究旨在评估不同形式的锌补充剂（有机形式的蛋氨酸锌（Zn-Met）、纳米形式的纳米氧化锌（nano ZnO）作为间接减少其对环境影响的方式，以及无机形式的硫酸锌（Zn-S））对处于严重热应激状态下的雄性兔子的生理参数、精液质量、抗氧化状态和激素谱的影响。36 只 V 型公兔（6-7 个月龄，体重 2842.29±34.46g）随机分为 4 组，第 1 组（对照组，Con）饲喂基础日粮且不添加锌，第 2、3 和 4 组分别饲喂基础日粮并添加 30mg/kg 的蛋氨酸锌（Zn-Met）、纳米氧化锌（ZnO-NPs）和硫酸锌（Zn-S），持续 10 周，这 3 组公兔遭受严重的环境环境温度（超过 32 的温湿度指数，THI）热应激。收集精液样本并评估体积、pH 值、活力、浓度、活力、初始果糖和精液血浆抗氧化浓度。测量血清中的肝功能、肾功能、激素和抗氧化谱。结果表明，与对照组相比，所有形式的锌补充剂均显著改善了热应激公兔的肾功能（肌酐）、血清抗氧化剂（SOD 和 CAT）、生理参数，尤其是在实验的第 1 个月，以及精液血浆抗氧化剂（SOD 和 CAT）。同样，纳米氧化锌（ZnO-NPs）补充剂显著提高了精子浓度、精子活力和初始果糖（P≤0.05）。蛋氨酸锌（Zn-Met）补充剂显著改善了肝功能并降低了精液 TBARs。与其他组相比，Zn-Met 和 ZnO-NPs 处理组提高了精液 TAC 和血液睾酮水平，降低了血液皮质醇水平。在推荐剂量（30mg/kg 日粮）下添加研究形式的 Zn-Met 和 ZnO-NPs 可缓解严重热应激，改善精液质量和抗氧化状态。
