Ghazi Narges, Saghravanian Nasrollah, Saeedi Pooya, Maboudinezhad Mohammad Mahdi
Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
Clin Exp Dent Res. 2025 Feb;11(1):e70080. doi: 10.1002/cre2.70080.
Tongue squamous cell carcinoma (TSCC) is the most prevalent oral cancer. Despite considerable advancements in treatment, the 5-year survival rate remains relatively unchanged. Langerhans cells (LCs) play an important role in antitumor immunity. Therefore, we attempt to evaluate the correlation between the LC count and disease prognosis.
Histopathologic slides from 24 cases, with at least 2 years of follow-up, were selected and categorized into early-stage (12 cases) and advanced-stage (12 cases) groups. An additional 12 slides of normal tissue comprised the control group. Immunohistochemical staining with the CD1a marker was performed to analyze the density of LCs. Statistical analysis assessed the impact of CD1a immune expression on patient survival and other variables such as age, gender, stage, and histopathological grade.
Comparison of CD1a+ cell counts across the three groups revealed a significant decrease in the advanced group. Furthermore, a lower count of CD1a+ cells correlated with poorer disease-free survival (DFS) (p < 0.001) and overall survival (OS) (p = 0.049). Although the CD1a+ cell count did not independently affect OS significantly (p = 0.210), it did show a significant impact on DFS as an independent variable (p = 0.002).
The significant correlation between CD1a expression and patients' prognosis and survival rates suggests that CD1a+ cells could serve as a crucial prognostic factor in the management and treatment of TSCC.
舌鳞状细胞癌（TSCC）是最常见的口腔癌。尽管在治疗方面取得了显著进展，但其5年生存率仍相对未变。朗格汉斯细胞（LCs）在抗肿瘤免疫中发挥重要作用。因此，我们试图评估LC计数与疾病预后之间的相关性。
选取24例有至少2年随访期的组织病理学切片，分为早期组（12例）和晚期组（12例）。另外12张正常组织切片作为对照组。采用CD1a标记进行免疫组化染色，分析LCs的密度。统计分析评估CD1a免疫表达对患者生存以及年龄、性别、分期和组织病理学分级等其他变量的影响。
三组间CD1a +细胞计数比较显示，晚期组显著减少。此外，CD1a +细胞计数较低与无病生存率（DFS）较差（p < 0.001）和总生存率（OS）较差（p = 0.049）相关。虽然CD1a +细胞计数对OS没有显著的独立影响（p = 0.210），但作为自变量对DFS有显著影响（p = 0.002）。
CD1a表达与患者预后和生存率之间的显著相关性表明，CD1a +细胞可作为TSCC管理和治疗中的关键预后因素。
