Bilgiseven Irem Mukaddes, Deveci Ilyas, Kismet Kemal, Karakurt Serdar
Department of Biochemistry, Science Faculty, Selcuk University, Konya, Türkiye.
Chemistry and Chemical Processing Technology Department, Technical Sciences Vocational School, Konya Technical University, Konya, Türkiye.
J Biomed Mater Res B Appl Biomater. 2025 Feb;113(2):e35544. doi: 10.1002/jbm.b.35544.
Wounds, disruptions in normal anatomy, are classified as acute or chronic. The choice of wound treatment relies significantly on dressing materials. Electrospun nanofibrous materials offer promising applications in wound healing, featuring a substantial surface area, close mimicry of the natural extracellular matrix, and adjustable water resistance, air permeability, and drug release. This research endeavors to formulate an innovative three-layered nanofibrous wound dressing using the electrospinning technique with the primary objectives of enhancing patient well-being, exhibiting antimicrobial characteristics, and expediting wound healing. The designed dressing comprises nanofibers of polyurethane (PU), quercetin (Q)-loaded polyethylene glycol (PEG), polyvinyl alcohol (PVA), and gelatin. Characterization of individual layers and the integrated wound dressing was conducted through SEM and FT-IR analyses. The efficacy of the nanofibrous wound dressing was assessed through in vitro human cell culture and in vivo rat wound models. The anti-toxic effects of nanofiber wound dressing on human epithelial and keratin cells have been proven. In vitro wound models in 24-well plates were utilized to assess the impact on wound healing rates. Photographic documentation of wound closure was performed at the different treatment hours, revealing complete closure of the wounds by the end of the 48th hour. Rats with 2 × 1 cm wounds were treated with the nanofibrous dressings, and wound healing progress was observed over a 14-day period. qRT-PCR was employed to analyze MMP-9, TIMP1, COL1A1, PDGFA, and VEGFC mRNA expressions. With its contemporary design surpassing existing treatments, the nanofiber wound dressing stands out for its wound-healing acceleration and antibacterial properties.
伤口，即正常解剖结构的破坏，可分为急性或慢性。伤口治疗方法的选择在很大程度上依赖于敷料材料。电纺纳米纤维材料在伤口愈合方面具有广阔的应用前景，其具有较大的表面积、能紧密模拟天然细胞外基质，且耐水性、透气性和药物释放均可调节。本研究致力于采用电纺技术制备一种创新的三层纳米纤维伤口敷料，主要目标是提高患者的舒适度、展现抗菌特性并加速伤口愈合。所设计的敷料包含聚氨酯（PU）、载有槲皮素（Q）的聚乙二醇（PEG）、聚乙烯醇（PVA）和明胶的纳米纤维。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT - IR）分析对各层以及整体伤口敷料进行了表征。通过体外人体细胞培养和体内大鼠伤口模型评估了纳米纤维伤口敷料的功效。已证实纳米纤维伤口敷料对人上皮细胞和角质形成细胞具有抗毒性作用。利用24孔板中的体外伤口模型评估对伤口愈合速率的影响。在不同治疗时间对伤口闭合情况进行拍照记录，结果显示在第48小时结束时伤口完全闭合。对有2×1厘米伤口的大鼠用纳米纤维敷料进行治疗，并在14天内观察伤口愈合进展。采用定量逆转录聚合酶链反应（qRT - PCR）分析基质金属蛋白酶 - 9（MMP - 9）、金属蛋白酶组织抑制剂1（TIMP1）、Ⅰ型胶原蛋白α1链（COL1A1）、血小板衍生生长因子A（PDGFA）和血管内皮生长因子C（VEGFC）的信使核糖核酸（mRNA）表达。这种纳米纤维伤口敷料以其超越现有治疗方法的现代设计，在加速伤口愈合和抗菌性能方面脱颖而出。
