Adepoju Temilola E, Fisher Hayden B, Winston Chloe, White Brian R
J Opt Soc Am A Opt Image Sci Vis. 2024 Oct 1;41(10):1942-1947. doi: 10.1364/JOSAA.531568.
Functional neuroimaging with widefield optical imaging (WOI) is potentially useful for studying developmental disorders in juvenile mice. However, WOI requires an intact-skull cranial window, and the effects of such windows on young mice are unknown. We performed intact-skull cranial window placement on mice as young as P7 to study the effects of chronic placement. Cranial windows placed at young ages (P7 and P10) were not longitudinally stable, resulting in significant attrition. Windows placed at ages P14 or less resulted in significant impairment to skull growth, which in turn caused artifacts in resting-state functional connectivity analysis. Longitudinal cranial windows should likely be avoided under P30.
利用宽场光学成像（WOI）进行功能神经成像可能有助于研究幼年小鼠的发育障碍。然而，WOI需要完整颅骨的颅窗，而这种颅窗对幼鼠的影响尚不清楚。我们对年仅P7的小鼠进行了完整颅骨颅窗植入，以研究长期植入的影响。在幼年（P7和P10）植入的颅窗纵向不稳定，导致显著损耗。在P14及以下年龄植入的颅窗会对颅骨生长造成显著损害，进而在静息态功能连接分析中产生伪影。30日龄以下的小鼠可能应避免使用纵向颅窗。
