Human-Induced Pluripotent Stem Cell-Based Alveolus-on-Chip Model to Study Influenza Virus A Infection.

This chapter introduces an alveolus-on-chip model for studying influenza virus A infection. The model represents a physiologically relevant alternative to animal models and a scalable and cost-effective approach to gaining mechanistic insights into human-related viral infection processes in vitro. We provide a comprehensive protocol for creating a human autologous model from human-induced pluripotent stem cell (hiPSC)-derived alveolar type II cells, endothelial cells, and macrophages. The protocol includes details on on-chip cell culture, microfluidic perfusion, and monitoring influenza A virus infection through immunofluorescence imaging.