Watanabe Takayuki, Ugajin Atsushi, Tateishi Kosuke, Watanabe Hidehiro, Mizunami Makoto
Research Center for Integrative Evolutionary Science, The Graduate University for Advanced Studies, Shonan Village, Hayama, 240-0193, Kanagawa, Japan.
JT Biohistory Research Hall, Takatsuki, 569-1125, Japan.
Sci Rep. 2025 Jan 31;15(1):3949. doi: 10.1038/s41598-025-87978-6.
Periplaneta cockroaches use periplanone analogs as female sex pheromones to attract males. We previously identified two periplanone receptor genes, PameOR1 and PameOR2, in the American cockroach Periplaneta americana. Here, we report the identification of PameOR1-like, an additional olfactory receptor resembling PameOR1 in P. americana. PameOR1-like showed high-level sequence similarity to PameOR1, and is preferentially expressed in the male antennae. Quantitative expression analysis revealed that, in the adult male antennae, the expression level of PameOR1 is 2.4-fold higher than that of PameOR1-like. Fluorescent in situ hybridization revealed that PameOR1 and PameOR1-like are co-expressed in the periplanone-A-responsive sensory neurons within the single walled-B sensilla on the male adult antennae. These data support the idea that PameOR1 and PameOR1-like are generated by a recent gene duplication event and play a redundant function in sex pheromone reception in P. americana.
蜚蠊利用类保幼激素类似物作为雌性性信息素吸引雄性。我们之前在美国蟑螂（美洲大蠊）中鉴定出两个保幼激素受体基因，即PameOR1和PameOR2。在此，我们报告了在美洲大蠊中鉴定出的一种类似于PameOR1的额外嗅觉受体PameOR1-like。PameOR1-like与PameOR1具有高度的序列相似性，且在雄性触角中优先表达。定量表达分析显示，在成年雄性触角中，PameOR1的表达水平比PameOR1-like高2.4倍。荧光原位杂交显示，PameOR1和PameOR1-like在成年雄性触角上单壁B型感器内对保幼激素A有反应的感觉神经元中共表达。这些数据支持了PameOR1和PameOR1-like是由最近的基因复制事件产生的，并且在美洲大蠊的性信息素接收中发挥冗余功能的观点。
