Tateishi Kosuke, Watanabe Takayuki, Nishino Hiroshi, Mizunami Makoto, Watanabe Hidehiro
Department of Earth System Science, Fukuoka University, Fukuoka 814-0180, Japan.
Research Center for Integrative Evolutionary Science, School of Advanced Sciences, Sokendai-Hayama, Shonan Village, Hayama 240-0193, Japan.
iScience. 2022 Apr 20;25(5):104272. doi: 10.1016/j.isci.2022.104272. eCollection 2022 May 20.
Insects detect odors via a large variety of odorant receptors (ORs) expressed in olfactory sensory neurons (OSNs). The insect OR is a heteromeric complex composed of a ligand-specific receptor and the co-receptor (ORco). In this study, we identified the gene of the cockroach, (), and performed RNAi-based functional analysis of . All OSNs in the basiconic sensilla expressed ORco and received a large variety of odors including sex pheromones. In trichoid sensilla, a ORco-positive OSN was consistently paired with a ORco-negative OSN tuned to acids. In adult cockroaches injected with dsRNA at the nymphal stage, the expression of , odor receptions via ORs, and its central processing were strongly suppressed. These results provide new insights into the molecular basis of olfactory reception in the cockroach. The long-lasting and irreversible effects of RNAi would be an effective method for controlling the household pest.
昆虫通过嗅觉感觉神经元（OSNs）中表达的多种气味受体（ORs）来检测气味。昆虫OR是一种异源复合物，由配体特异性受体和共同受体（ORco）组成。在本研究中，我们鉴定了蟑螂的基因（），并对进行了基于RNA干扰的功能分析。锥形感器中的所有OSNs均表达ORco，并能接收包括性信息素在内的多种气味。在毛形感器中，一个ORco阳性的OSN始终与一个对酸有反应的ORco阴性的OSN配对。在若虫阶段注射dsRNA的成年蟑螂中，的表达、通过ORs的气味接收及其中枢处理受到强烈抑制。这些结果为蟑螂嗅觉接收的分子基础提供了新的见解。RNA干扰的持久和不可逆作用将是控制这种家庭害虫的有效方法。
