Yang Zhi, Lin Shuxuan, Wang Hai, Zhou Jiong, Lin Hong, Zhou Jian
College of Eco-Environmental Engineering, Guizhou Minzu University, Guiyang, 550025, China.
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing, 400045, China.
J Environ Manage. 2025 Feb;375:124200. doi: 10.1016/j.jenvman.2025.124200. Epub 2025 Jan 31.
Simultaneous partial nitrification-denitrification (SPND) is a promising process for nitrogen (N) removal from kitchen digested wastewater characterized by a low C/N ratio. However, its widespread application is often restricted due to the unstable partial nitrification and unsatisfactory denitrification performance. This work developed a novel simultaneous partial nitrification, endogenous and autotrophic denitrification process using a single-stage electrolysis-integrated sequencing batch biofilm reactor (E-SBBR) with anoxic/electro-anaerobic/aerobic operating strategy. The novel process considerably enhanced the stability and N removal efficiency (NH-N>94.5% and TN>90.8%) of the SPND process. The pre-electro-anaerobic phase achieved alkalinity and H generation, and intracellular carbon storage. The increased alkalinity resulted in increased free ammonia (FA) which secured complete suppression of nitrite-oxidizing bacteria (NOB). SPND efficiency in the aerobic phase was dramatically improved using polyhydroxyalkanoates (PHAs) and H as electron donors for endogenous and autotrophic denitrification. Microbial community analysis indicated the successful washout of NOB and the enrichment of ammonia-oxidizing bacteria (AOB), denitrifying glycogen accumulating organisms (DGAOs), autotrophic and heterotrophic denitrifiers in the system. This research presents a distinctive SPND process for intensified kitchen digested wastewater treatment and gives insights into the underlying mechanism.
同步短程硝化反硝化（SPND）是一种从低C/N比的厨房消化废水中去除氮的很有前景的工艺。然而，由于部分硝化不稳定和反硝化性能不理想，其广泛应用常常受到限制。本研究开发了一种新型的同步短程硝化、内源和自养反硝化工艺，该工艺采用单级电解集成序批式生物膜反应器（E-SBBR），采用缺氧/电厌氧/好氧运行策略。该新工艺显著提高了SPND工艺的稳定性和脱氮效率（NH-N>94.5%，TN>90.8%）。预电厌氧阶段实现了碱度和氢气的产生以及细胞内碳的储存。碱度的增加导致游离氨（FA）增加，从而确保完全抑制亚硝酸盐氧化细菌（NOB）。在好氧阶段，利用聚羟基脂肪酸酯（PHA）和氢气作为内源和自养反硝化的电子供体，显著提高了SPND效率。微生物群落分析表明，系统中成功去除了NOB，并富集了氨氧化细菌（AOB）、反硝化糖原积累菌（DGAO）、自养和异养反硝化菌。本研究提出了一种独特的用于强化厨房消化废水处理的SPND工艺，并深入探讨了其潜在机制。
