Piedra Valeria, Usaga Jessie, Redondo-Solano Mauricio, Uribe-Lorío Lidieth, Valenzuela-Martínez Carol, Barboza Natalia
Food Technology Department, University of Costa Rica, San Pedro.
National Center of Food Science and Technology, University of Costa Rica, San Pedro.
Ital J Food Saf. 2024 Dec 2;14(1):12494. doi: 10.4081/ijfs.2024.12494.
This study aimed to characterize lactic acid bacteria (LAB) isolated from Costa Rican agro-industrial waste and explore their bioprotective potential against Salmonella in yogurt. A total of 43 LAB isolates were identified using the 16S rRNA region. In vitro inhibition of Salmonella, Listeria monocytogenes, Staphylococcus aureus, and Escherichia coli was determined. A total of 15 of the 43 isolates showed a good to strong antimicrobial effect against at least two pathogens. A total of 14 selected isolates were evaluated for antibiotic resistance, gelatinase, and hemolytic activity. The bioprotective effect of the most promising strain, Lactiplantibacillus pentosus, was assessed against Salmonella sp. during yogurt fermentation. All the isolates were resistant to vancomycin and showed variable degrees of susceptibility to other antibiotics. All of the isolates were negative for gelatinase, and 5 isolates had no hemolytic activity. A significant inhibitory effect of L. pentosus_58(6)-2I (p<0.05) against Salmonella during fermentation was found, but pathogen reduction was limited to 0.611 log CFU/mL.
本研究旨在对从哥斯达黎加农产品加工废弃物中分离出的乳酸菌（LAB）进行特性分析，并探索其在酸奶中对沙门氏菌的生物保护潜力。使用16S rRNA区域对总共43株乳酸菌分离株进行了鉴定。测定了其对沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和大肠杆菌的体外抑制作用。43株分离株中共有15株对至少两种病原体表现出良好至强烈的抗菌作用。对总共14株选定的分离株进行了抗生素抗性、明胶酶和溶血活性评估。在酸奶发酵过程中，评估了最有前景的菌株戊糖乳杆菌对沙门氏菌的生物保护作用。所有分离株对万古霉素耐药，对其他抗生素表现出不同程度的敏感性。所有分离株的明胶酶检测均为阴性，5株分离株无溶血活性。发现戊糖乳杆菌_58(6)-2I在发酵过程中对沙门氏菌有显著抑制作用（p<0.05），但病原体减少量仅限于0.611 log CFU/mL。
